Annals of the Russian academy of medical sciencesAnnals of the Russian academy of medical sciencesВестник Российской академии медицинских наук0869-60472414-3545"Paediatrician" Publishers LLC16710.15690/vramn.v68i7.711PEDIATRICS: CURRENT ISSUESАКТУАЛЬНЫЕ ВОПРОСЫ ПЕДИАТРИИIMMUNOLOGIC INDEXES, ENZYME STATUS OF LYMPHOCYTES AND FUNCTIONAL ACTIVITY OF BLOOD NEUTROPHILS IN CHILDREN WITH INFECTIOUS MONONUCLEOSIS CAUSED BY EPSTEIN–BARR VIRUSИММУНОЛОГИЧЕСКИЕ ПОКАЗАТЕЛИ, ФЕРМЕНТНЫЙ ПРОФИЛЬ ЛИМФОЦИТОВ И ФУНКЦИОНАЛЬНАЯ АКТИВНОСТЬ НЕЙТРОФИЛОВ КРОВИ У ДЕТЕЙ С ИНФЕКЦИОННЫМ МОНОНУКЛЕОЗОМ, ВЫЗВАННЫМ ВИРУСОМ ЭПШТЕЙНА–БАРРKurtasovaL. M.КуртасоваЛ. М.
Russian Federation

PhD, Professor, Department of Clinical Immunology, Krasnoyarsk State Medical University named after Prof. V. F. Voyno-Yasenetsky Address: 660022, Krasnoyarsk, Partizan Zheleznyaka St., 1/1; tel.: (391) 227-24-13

office@aids.krsn.ruTolstikovaA. E.ТолстиковаА. Е.
Russian Federation

PhD student for the Degree of Candidate of Biological Sciences, Medical Research Institute for Northern Problems Siberian Branch of RAMS Address: 660022, Krasnoyarsk, Partizan Zheleznyaka St., 3G; tel.: (391) 212-52-63

a-golovanova@mail.ruSavchenkoA. A.СавченкоА. А.
Russian Federation

PhD, Professor, Head of the Molecule-cellular Physiology and Pathology Laboratory, Medical Research Institute for Northern Problems Siberian Branch of RAMS Address: 660022, Krasnoyarsk, Partizan Zheleznyaka St., 3G; tel.: (391) 212-52-63

aasavchenko@yandex.ruVoyno-Yasenetsky Krasnoyarsk State Medical University, Russian FederationКрасноярский государственный медицинский университет им. проф. В.Ф. Войно-Ясенецкого, Российская ФедерацияMedical Research Institute for Northern Problems of Siberian Division of RAMS, Krasnoyarsk, Russian FederationНИИ медицинских проблем Севера СО РАМН, Красноярск, Российская Федерация19072013687Vestnik Rossiiskoi akademii medetsinskikh nauk / Annals of the Russian academy of medical sciencesВестник Российской академии медицинских наук424607082015Copyright ©; 1970, "Paediatrician" Publishers LLCCopyright ©; 1970, Издательство "Педиатръ"1970"Paediatrician" Publishers LLCИздательство "Педиатръ"https://vestnikramn.spr-journal.ru/jour/about/submissionshttps://vestnikramn.spr-journal.ru/jour/article/view/167

Aim. Explore the immunological parameters, levels of activity of NAD(P)-dependent dehydrogenases lymphocytes, interferon status parameters, phagocytic activity and chemiluminescence response of neutrophils in the blood of children in the acute phase of infectious mononucleosis caused by the Epstein-Barr virus. Patients and methods. 65 children at the age of 4–6 years old with infectious mononucleosis caused by EBV in acute phase were observed. Such indexes as cell-mediated, humoral and interferon immunity, NAD(P)-depended dehydrogenases activity in blood lymphocyte, phagocytes activity, levels of spontaneous and induced chemiluminescence of peripheral blood neutrophils were studied. Results. Children with EVB-infection have immunophenotype spectrum changes and changes of enzymes status of blood lymphocytes against the increasing in leucocytes and the useful increasing in lymphocytes. The useful increasing in IgA, IgM, IgG contenting in serum blood were found. The decreasing of spontaneous production of IFN α and the decreasing of induced production of IFN α, IFN γ were determined. The breach of phagocytes activity and chemiluminescent response of blood neutrophils were found. Conclusions. The children in the acute phase of infectious mononucleosis caused by the Epstein-Barr virus, there are changes in the immune status, changes the activity of NAD(P)-dependent dehydrogenases in blood lymphocytes, marked changes in functional and metabolic state of peripheral blood neutrophils.

Цель исследования: изучить иммунологические показатели, уровни активности НАД(Ф)-зависимых дегидрогеназ лимфоцитов, параметры интерферонового статуса, фагоцитарной активности и хемилюминесцентного ответа нейтрофилов крови у детей в острый период инфекционного мононуклеоза, вызванного вирусом Эпштейна–Барр. Пациенты и методы. Обследованы 65 детей в возрасте 4–6 лет в острый период инфекционного мононуклеоза, вызванного вирусом Эпштейна–Барр. Изучены показатели клеточного, гуморального и интерферонового звена иммунитета, уровень активности НАД(Ф)-зависимых дегидрогеназ лимфоцитов крови, параметры фагоцитарной активности, спонтанной и индуцированной хемилюминесценции нейтрофилов периферической крови. Результаты. У детей с ВЭБ-инфекцией на фоне лейкоцитоза и выраженного лимфоцитоза отмечаются изменения иммунофенотипического спектра и энзиматического статуса лимфоцитов периферической крови. Установлено повышение концентрации IgA, M и G в сыворотке крови. Обнаружено снижение спонтанной продукции IFN α и уменьшение индуцированной выработки IFN α и γ мононуклеарами крови. Установлены нарушения фагоцитарной активности и хемилюминесцентного ответа нейтрофилов крови. Выводы. У детей в острый период инфекционного мононуклеоза, вызванного вирусом Эпштейна-Барр, наблюдаются изменения в иммунном статусе, изменяется активность НАД(Ф)-зависимых дегидрогеназ лимфоцитов крови, отмечаются изменения функционально-метаболического состояния нейтрофилов периферической крови.

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