Annals of the Russian academy of medical sciencesAnnals of the Russian academy of medical sciencesВестник Российской академии медицинских наук0869-60472414-3545"Paediatrician" Publishers LLC16110.15690/vramn.v68i7.705ANDROLOGY: CURRENT ISSUESАКТУАЛЬНЫЕ ВОПРОСЫ АНДРОЛОГИИGLUTATHION AS AN IMPORTANT COMPONENT OF THIOSULFID SYSTEM OF INFERTILITY PATHOGENESIS IN OVERWEIGHT MENГЛУТАТИОН КАК ВАЖНЕЙШИЙ КОМПОНЕНТ ТИОЛДИСУЛЬФИДНОЙ СИСТЕМЫ В ПАТОГЕНЕЗЕ БЕСПЛОДИЯ МУЖЧИН С ИЗБЫТОЧНОЙ МАССОЙ ТЕЛАKolesnikovaL. I.КолесниковаЛ. И.
Russian Federation

PhD, Professor, RAMS academician, Director of the Scientific Center of Family Health and Human Reproduction Problems, Siberian Branch of Russian Academy of Medical Sciences Address: 664003, Irkutsk, Timiryazeva Street, 16; tel.: (3952) 20-76-36, 20-73-67

iphr@sbamsr.irk.ruVanteevaO. A.ВантееваО. А.
Russian Federation

PhD student, Scientific Center of Family Health and Human Reproduction Problems, Siberian Branch of Russian Academy of Medical Sciences Address: 664003, Irkutsk, Timiryazeva Street, 16; tel.: (3952) 20-76-36

olga_tolpygina@mail.ruKurashovaN. A.КурашоваН. А.
Russian Federation

PhD, Research Worker, Scientific Center of Family Health and Human Reproduction Problems, Siberian Branch of Russian Academy of Medical Sciences Address: 664003, Irkutsk, Timiryazeva Street, 16; tel.: (3952) 20-76-36

nakurashova@yandex.ruVlasovB. Ya.ВласовБ. Я.
Russian Federation

PhD, Professor, Senior Research Worker, Laboratory of Physiology and Pathology of endocrine system, Scientific Center of Family Health and Human Reproduction Problems, Siberian Branch of Russian Academy of Medical Sciences Address: 664003, Irkutsk, Timiryazeva Street, 16; tel.: (3952) 20-76-36, 20-73-67

iphr@sbamsr.irk.ruScientific Center of the Problems of Family Health and Human Reproduction, Irkutsk, Russian FederationНаучный центр проблем здоровья семьи и репродукции человека, Иркутск, Российская Федерация19072013687Vestnik Rossiiskoi akademii medetsinskikh nauk / Annals of the Russian academy of medical sciencesВестник Российской академии медицинских наук91207082015Copyright ©; 1970, "Paediatrician" Publishers LLCCopyright ©; 1970, Издательство "Педиатръ"1970"Paediatrician" Publishers LLCИздательство "Педиатръ"https://vestnikramn.spr-journal.ru/jour/about/submissionshttps://vestnikramn.spr-journal.ru/jour/article/view/161

Aim: to study the glutathionic part of thiosulfid system and oxidative stress in infertile men with different body mass index. Patients and methods: examined 60 infertile men with normal and overweight, and 40 healthy men with proven fertility. Active components of the antioxidant defense system of the body was assessed by the level of reduced and oxidized glutathione by the method of PY Hissin, determination of TBA-active products was carried out by the method VB Gavrilova. In the analysis of inter-group differences for independent samples was used parametric Student criterion. Results: the oxidative stress in obese men was characterized by significant elevation of oxidized form of glutathione (GSSG) in comparison with its reduced form. We discussed our results in the context of GSSG «quantivalence» to numerous of cellular processes in the setting of metabolic disorders in infertile and obese men. The ratio of reduced glutathione to oxidized infertile men decreased by 1,5 times in patients with obesity and 1,2 times in those with normal body mass index. Conclusions: Glutathione is the most important link in the study of oxidative stress in men with infertility. Raising the level of oxidized glutathione is due to the presence of his additional functions that are implemented at higher values of body mass index, especially in association with infertility.

Цель исследования: изучить состояние глутатионового звена тиолдисульфидной системы у мужчин с бесплодием с различными индексами массы тела на фоне окислительного стресса. Пациенты и методы: обследовано 60 мужчин с бесплодием с нормальной и избыточной массой тела и 40 практически здоровых мужчин с доказанной фертильностью. Активность компонентов системы антиоксидантной защиты организма оценивалась по уровню восстановленного и окисленного глутатиона по методу P.Y. Hissin, определение содержания ТБК-активных продуктов осуществляли по методу В.Б. Гаврилова. При анализе межгрупповых различий для независимых выборок использовали параметрический критерий Стьюдента. Результаты: у мужчин с бесплодием и избыточной массой тела окислительный стресс характеризовался статистически значимым повышением концентрации окисленной формы глутатионa (GSSG) по сравнению с ее восстановленной формой. Полученные результаты обсуждаются в связи с «поливалентностью» GSSG по отношению ко многим клеточным процессам, которая проявляется специфическим образом на фоне серьезной дисрегуляции метаболизма у бесплодных мужчин с высокой массой тела. Выводы: глутатион является важнейшим звеном при изучении окислительного стресса у мужчин с бесплодием. Повышение уровня окисленного глутатиона связано с наличием у него дополнительных функций, которые реализуются при повышении величины индекса массы тела, особенно в ассоциации с бесплодием.

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