Annals of the Russian academy of medical sciencesAnnals of the Russian academy of medical sciencesВестник Российской академии медицинских наук0869-60472414-3545"Paediatrician" Publishers LLC14910.15690/vramn.v68i9.779SHORT MESSAGESКРАТКИЕ СООБЩЕНИЯRECENT ADVANCES IN THE STUDY OF THE STEM CELLS MIGRATION METHODSСОВРЕМЕННЫЕ ДОСТИЖЕНИЯ В СОЗДАНИИ МЕТОДОВ ИЗУЧЕНИЯ МИГРАЦИИ СТВОЛОВЫХ КЛЕТОКPoveshchenkoA. F.ПовещенкоА. Ф.
Russian Federation

PhD, Head of the laboratory of protective system physiology of the Federal State Budgetary Institution «Scientific Research Institute of clinical and experimental lymphology» under the Siberian Branch of RAMS. Address: 2, Timakova St., Novosibirsk, 630117; tel.: (383) 333-64-09

poveshchenkoa200@mail.ruPoveshchenkoO. V.ПовещенкоО. В.
Russian Federation

MD, Head of the lymphotropic therapy and lymphodiagnostic laboratory of the Federal State Budgetary Institution «Scientific Research Institute of clinical and experimental lymphology» under the Siberian Branch of RAMS. Address: 2, Timakova St., Novosibirsk, 630117; tel.: (383) 333-64-09

poveshchenkoa200@mail.ruKonenkovV. I.КоненковВ. И.
Russian Federation

PhD, professor, member of the RAMS, director of the Federal State Budgetary Institution «Scientific Research Institute of clinical and experimental lymphology» under the Siberian Branch of RAMS. Address: 2, Timakova St., Novosibirsk, 630117; tel.: (383) 333-64-09

lymphology@soramn.ruScientific Institution of Clinical and Experimental Lymphology of the Siberian Branch, RAMS, Novosibirsk, Russian FederationНИИ клинической и экспериментальной лимфологии СО РАМН, Новосибирск, Российская Федерация10092013689Vestnik Rossiiskoi akademii medetsinskikh nauk / Annals of the Russian academy of medical sciencesВестник Российской академии медицинских наук465107082015Copyright ©; 1970, "Paediatrician" Publishers LLCCopyright ©; 1970, Издательство "Педиатръ"1970"Paediatrician" Publishers LLCИздательство "Педиатръ"https://vestnikramn.spr-journal.ru/jour/about/submissionshttps://vestnikramn.spr-journal.ru/jour/article/view/149

Review is devoted to a comparative analysis of stem cells migration methods for studying in experimental conditions and clinics, assessing their strengths and weaknesses. It is impossible to single out any one preferred method for studying the migration of stem cells. Each method has its own set of advantages and disadvantages. X-ray methods " suffer" from non-specific binding of contrast marks, followed by rapid degradation and the release of the body. If it is necessary to study the migration of the transplanted stem cells for a long time interval, the visualization of the reporter gene is the best available techniques, since it can provide a non-invasive real time monitoring of the location in the tissue and stem cells isolated help assess their viability and proliferation. The most attractive of the agents for monitoring - endogenous, i.e. natural constituents stem cells, for example, genetic markers. Criteria for the application of methods to study migration in clinical and experimental results have significant differences. The main criteria for the methods of the study of cell migration in clinical settings, it must be biocompatible, safe, non-toxic, non-invasive. Accordingly, the main criteria for the experimental methods for studying the migration will be accuracy, the ability to quantify the long-term migration of cells and stability of the "label".

Обзор посвящен сравнительному анализу методов исследования миграции стволовых клеток в условиях эксперимента и клиники, оценке их преимуществ и недостатков. Невозможно выделить какой-то один преимущественный метод исследования миграции стволовых клеток, идеальный во всех аспектах. Каждый из методов имеет свой набор достоинств и недостатков. Рентгенологические методы «страдают» неспецифическим связыванием контрастной метки с последующей быстрой деградацией и выделением из организма. Если необходимо изучить миграцию трансплантированных стволовых клеток в течение длительного временного интервала, то визуализация гена-репортера является наилучшим из имеющихся методов, поскольку она может обеспечить неинвазивный мониторинг в реальном времени расположения в тканях организма единичных стволовых клеток и помочь оценить их жизнеспособность и пролиферацию. Наиболее привлекательны из агентов для отслеживания — эндогенные, т.е. природные составляющие стволовых клеток, например, генетические маркеры. Критерии для применения методов изучения миграции в условиях клиники и эксперимента имеют существенные различия. Главными критериями для методов исследования миграции клеток в клинических условиях, очевидно, должны быть биосовместимость, безопасность, нетоксичность, неинвазивность. Соответственно, основными критериями для экспериментальных методов изучения миграции будут точность, возможность длительной количественной оценки миграции клеток и стабильность «метки».

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