Annals of the Russian academy of medical sciencesAnnals of the Russian academy of medical sciencesВестник Российской академии медицинских наук0869-60472414-3545"Paediatrician" Publishers LLC14310.15690/vramn.v68i9.773MOLECULAR MEDICINE AND GENETICS: CURRENT ISSUESАКТУАЛЬНЫЕ ВОПРОСЫ ГЕНЕТИКИ И МОЛЕКУЛЯРНОЙ МЕДИЦИНЫAGE AND SEXUAL CHANGES STRUCTURE OF GENES CYTOKINES NETWORKS IN RUSSIAN POPULATIONВОЗРАСТНАЯ И ПОЛОВАЯ ДИНАМИКА СТРУКТУРЫ ЦИТОКИНОВЫХ ГЕННЫХ СЕТЕЙ НАСЕЛЕНИЯ РОССИИKonenkovV. I.КоненковВ. И.
Russian Federation

PhD, professor, member of RAMS, Head of the Clinical Immunogenetics Laboratory, director of the Scientific Research Institute of clinical and experimental lymphology under the Siberian Branch of RAMS. Address: 2, Timakova St., Novosibirsk, 630117, tel.: (383) 227-01-94

konenkov@soramn.ruProkof'evV. F.ПрокофьевВ. Ф.
Russian Federation

MD, leading research scientist of the Clinical Immunogenetics Laboratory of the Scientific Research Institute of clinical and experimental lymphology under the Siberian Branch of RAMS. Address: 2, Timakova St., Novosibirsk, 630117, tel.: (383) 227-01-94

vprok@ngs.ruShevchenkoA. V.ШевченкоА. В.
Russian Federation

MD, senior research scientist of the Clinical Immunogenetics Laboratory of the Scientific Research Institute of clinical and experimental lymphology under the Siberian Branch of RAMS. Address: 2, Timakova St., Novosibirsk, 630117, tel.: (383) 227-01-94

shalla64@mail.ruChernyavskiiA. M.ЧернявскийА. М.
Russian Federation

PhD, professor, Head of the Center of Aortic Surgery, coronary and peripheral arteries of the Federal State Budgetary Institution E. N. Meshalkin Scientific Research Institute of circulation pathology of The Ministry of Health of the Russian Federation. Address: 15, Rechkunovskaya St., Novosibirsk, 630055, tel.: (383) 332-47-58

tsvelo@mail.ruKaras'kovA. M.КараськовА. М.
Russian Federation

PhD, professor, member of RAMS, director of the Federal State Budgetary Institution E. N. Meshalkin Scientific Research Institute of circulation pathology of The Ministry of Health of the Russian Federation. Address: 15, Rechkunovskaya St., Novosibirsk, 630055, tel.: (383) 332-47-58

tsvelo@mail.ruScientific Research Institute of Clinical And Experimental Limfology RAMS, Novosibirsk, Russian FederationНИИ клинической и экспериментальной лимфологии СО РАМН, Новосибирск, Российская ФедерацияScientific Research Institute of a Pathology of Blood Circulation named after E.N. Meshalkin of the Health Ministries of Russian Federation, Novosibirsk, Russian FederationНИИ патологии кровообращения им. Е.Н. Мешалкина МЗ РФ, Новосибирск, Российская Федерация10092013689Vestnik Rossiiskoi akademii medetsinskikh nauk / Annals of the Russian academy of medical sciencesВестник Российской академии медицинских наук152107082015Copyright ©; 1970, "Paediatrician" Publishers LLCCopyright ©; 1970, Издательство "Педиатръ"1970"Paediatrician" Publishers LLCИздательство "Педиатръ"https://vestnikramn.spr-journal.ru/jour/about/submissionshttps://vestnikramn.spr-journal.ru/jour/article/view/143

Objective: to study frequencies of occurrence of the combined genetic attributes including different variants of cytokines genotypes (TNFA, IL1B, IL4, IL6, IL10, VEGF), in different on sexual and age groups in population of Siberia Caucasoid. Material and methods. Frequencies of distribution of variants of structure genes cytokines networks among 500 representatives of Siberia Caucasian population, men and women of two age groups - more younger than 35 years ("young") and 55 and more years ("elderly") are investigated. In structure of investigated genes cytokines net has come 10 variants of polymorphic sites of cytokines genes and vascular endothelial growth factor gene: TNFА-863 C→A, TNFА-308 G→A, TNFА-238 G→A, IL1B-31 С→T, IL4-590 С→T, IL6-174 G→C, IL10-1082 G → A, IL10-592 А→С, VEGF-2578 C→A and VEGF+936 С→T. Genotyping are carried out by restriction fragment length polymorphism method. Processing of results carried out on the basis of the original methodological approach including the complex connected computer analysis of genic circuits of various dimension. Results and conclusions. It is shown, that the significant part of variants genes cytokines networks, which widely distributed among young people is completely absent in the "elderly" age group. Such variants disappearing with age separately for men and women are established. At the program mathematical analysis it is established, that parameters of the odds ratio achieve two-place sizes (OR =27, p =0,0004), that testifies to high specificity of complex genetic attributes. Presence in genome such variants of genes cytokines networks , found out in the childhood or young age, as supposed, is unfavorable personalized prognostic factors of life span of the individual.

Цель исследования: изучить частоты встречаемости комбинированных генетических признаков, включающих различные варианты генотипов цитокинов (TNFA, IL1B, IL4, IL6, IL10, VEGF), в различных по полу и возрасту группах европеоидного населения Сибири. Материал и методы. Исследованы частоты распределения вариантов структуры цитокиновой генной сети среди 500 представителей европеоидного населения Сибири, мужчин и женщин двух возрастных групп - младше 35 лет («молодые») и 55 и более лет («пожилые»). В состав исследованной цитокиновой генной сети вошло 10 вариантов полиморфных участков генов цитокинов и факторов роста: TNFА-863 C→A, TNFА-308 G→A, TNFА-238 G→A, IL1B-31 С→T, IL4-590 С→T, IL6-174 G→C, IL10-1082 G→ A, IL10-592 А→С, VEGF-2578 C→A и VEGF+936 С→T. Генотипирование осуществляли методом рестриктивного анализа продуктов амплификации. Обработку результатов проводили на основе оригинального методологического подхода, включающего комплексный сопряженный компьютерный анализ генных цепей различной размерности. Результаты и выводы. Показано, что значительная часть широко распространенных среди молодых людей вариантов цитокиновой генной сети полностью отсутствует в старшей возрастной группе. Установлены варианты таких исчезающих с возрастом вариантов отдельно для мужчин и женщин. При программном математическом анализе установлено, что показатели отношения шансов достигают двузначных величин (OR =27, p =0,0004), что свидетельствует о высокой специфичности комплексных генетических признаков. Предполагается, что наличие в геноме таких вариантов цитокиновой генной сети, обнаруживаемых в детстве или молодом возрасте, является неблагоприятным персонализированным прогностическим признаком продолжительности жизни индивида.

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