Annals of the Russian academy of medical sciencesAnnals of the Russian academy of medical sciencesВестник Российской академии медицинских наук0869-60472414-3545"Paediatrician" Publishers LLC13510.15690/vramn.v68i10.784PEDIATRICS: CURRENT ISSUESАКТУАЛЬНЫЕ ВОПРОСЫ ПЕДИАТРИИNITRIC OXIDE, ITS METABOLITES, AND GLUTATHIONE SYSTEM IN CHILDREN WITH CHRONIC VIRAL HEPATITIS В AND СОКСИД АЗОТА, ЕГО МЕТАБОЛИТЫ И СИСТЕМА ГЛУТАТИОНА У ДЕТЕЙ С ХРОНИЧЕСКИМ ВИРУСНЫМ ГЕПАТИТОМ В И СKorkotashviliL. V.КоркоташвилиЛ. В.
Russian Federation

MD, Head of the laboratory diagnostic department of FSBI “Nizhny Novgorod Research Institute of Pediatric Gastroenterology” of the Ministry of Health of Russian Federation. Address: 22, Semashko St., Nizhny Novgorod, 603950, tel.: (831) 436-54-60

lvkor@inbox.ruRomanovaS. V.РомановаС. В.
Russian Federation

MD, leading research scientist of the upper alimentary tract pathology clinic of FSBI “Nizhny Novgorod Research Institute of Pediatric Gastroenterology” of the Ministry of Health of Russian Federation. Address: 22, Semashko St., Nizhny Novgorod, 603950, tel.: (831) 436-15-90

niidg@mail.ruKolesovS. A.КолесовС. А.
Russian Federation

MD, senior research scientist of the laboratory diagnostic department of FSBI “Nizhny Novgorod Research Institute of Pediatric Gastroenterology” of the Ministry of Health of Russian Federation. Address: 22, Semashko St., Nizhny Novgorod, 603950, tel.: (831) 436-54-60

niidg@mail.ruNizhny Novgorod Research Institute of Children Gastroenterology, Russian FederationНижегородский НИИ детской гастроэнтерологии, Российская Федерация101020136810Vestnik Rossiiskoi akademii medetsinskikh nauk / Annals of the Russian academy of medical sciencesВестник Российской академии медицинских наук263007082015Copyright ©; 1970, "Paediatrician" Publishers LLCCopyright ©; 1970, Издательство "Педиатръ"1970"Paediatrician" Publishers LLCИздательство "Педиатръ"https://vestnikramn.spr-journal.ru/jour/about/submissionshttps://vestnikramn.spr-journal.ru/jour/article/view/135

Aim. To detect changes in the concentration of nitric oxide and its metabolites – nitrites and nitrates in conjunction with the indices of the glutathione system in the children with chronic hepatitis В and С. Patients and methods. In the research took a part 76 patients (boys or girls) with chronic hepatitis В and С aged from 7 up to 16 years. Nitric oxide and its metabolites determined by method of V.A. Metel’skaya and N.G. Gumanova; level of reduced glutathione – the titrimetric analysis; activity of glutathione transferase – the method of spectrophotometry. Results. The content of nitric oxide and its metabolites– nitrites and nitrates – in serum and urine of children with chronic viral hepatitis В and С have increased. Correlations were revealed between nitric oxide and its metabolites in serum and urine, as well as between these indices and glutathione system parameters – reduced glutathione concentration and activity of enzymes of glutathione transferase of erythrocytes and plasma. Conclusions. In the children with chronic viral hepatitis В and С the increase of the concentration of nitric oxide and its metabolites in serum and urine is accompanied by decrease of reduced glutathione concentration and increase of glutathione transferase activity in erythrocytes and plasma. These findings suggest the presence of oxidative stress due to which the therapy should be strengthened by antioxidant effect.

Цель исследования. Выявить изменения концентрации окиси азота и его метаболитов – нитритов и нитратов во взаимосвязи с показателями системы глутатиона у детей с хроническим гепатитом В и С. Пациенты и методы. В исследовании приняли участие 76 детей с хроническим гепатитом В и С в возрасте от 7 до 16 лет обоего пола. Оксид азота и его метаболиты определяли по методу В.А. Метельской и Н.Г. Гумановой; уровень восстановленного глутатиона - титрометрическим методом; активность глутатионтрансферазы - методом спектрофотометрии. Результаты. Содержание оксида азота и его метаболитов — нитритов и нитратов ― в сыворотке крови и моче детей с хроническим вирусным гепатитом В и С повышено. Выявлены взаимосвязи между оксидом азота и его метаболитами в сыворотке крови и моче, а также между этими показателями и параметрами системы глутатиона ― концентрацией восстановленного глутатиона и активностью ферментов глутатионтрансферазы эритроцитов и плазмы. Выводы. У детей с хроническим вирусным гепатитом В и С повышение концентрации оксида азота и его метаболитов в сыворотке крови и моче сопровождается снижением концентрации восстановленного глутатиона и повышением активности глутатионтрансферазы эритроцитов и плазмы. Полученные результаты говорят о наличии окислительного стресса, в связи с чем необходимо оптимизировать терапию с усилением антиоксидантного воздействия.

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