ACELLULAR TRACHEAL CARTILAGINOUS SCAFFOLD PRODUCING FOR TISSUE-ENGINEERED CONSTRUCTS

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Abstract

Background: Tissue-engineered trachea transplantation remains the last chance for a variety of patients suffering from severe cicatricial tracheal stenosis. Despite the series of carried studies, the final solution hasn’t been found. Creating a functionally complete hyaline cartilage graft in vitro still presents a fundamental problem, and a number of researchers consider it as the key to a successful tracheal tissue-engineering.

Aims: The study aimed to investigate the capability of detergent complex and DNAse I for human tracheal cartilage decellularization in short-time exposition for acellular scaffold obtaining.

Materials and methods: Isolated from cadaveric trachea human native cartilage was used for decellularization by ensimatic-detergent complex including Triton X-100, DMSO, and DNAse I. The scaffold was characterised by histological examinations, analysis of the residual DNA content, and cell metabolic activity colorimetric test with culture in the scaffold fragments.

Results: The obtained scaffolds presented highly porous structure mostly composed of collagen and glycosaminoglycans with an insignificant residual DNA level, absence of citotoxicity, and capability for cell proliferative activity stimulation.

Conclusions: Thus, the study provides a new short-time technology for hyaline cartilage decellularization in order to achieve acellular scaffolds in step with the tissue engineering requirements.

About the authors

D. S. Baranovsky

Sechenov First Moscow State Medical University Ministry of Health of Russian Federation

Author for correspondence.
Email: doc.baranovsky@gmail.com
ORCID iD: 0000-0002-6154-9959

Научный сотрудник Института регенеративной медицины.

119991, Москва, ул. Трубецкая, д. 8, стр. 2.

SPIN-код: 6913-6361

Russian Federation

A. G. Demchenko

Sechenov First Moscow State Medical University Ministry of Health of Russian Federation

Email: demchenkoann@yandex.ru
ORCID iD: 0000-0002-4460-7627

Студент-лаборант 3-го курса Института регенеративной медицины.

119991, Москва, ул. Трубецкая, д. 8, стр. 2.

SPIN-код: 3779-9060

Russian Federation

R. V. Oganesyan

Sechenov First Moscow State Medical University Ministry of Health of Russian Federation

Email: oganesyan.rv@gmail.com
ORCID iD: 0000-0001-8967-5597

Студент 5-го курса Института регенеративной медицины.

119991, Москва, ул. Трубецкая, д. 8, стр. 2.

SPIN-код: 8106-3394

Russian Federation

G. V. Lebedev

Lomonosov Moscow State University

Email: lebedev.george12@gmail.com
ORCID iD: 0000-0001-8493-3390

Студент 2-го курса.

119991, Москва, Ломоносовский проспект, д. 1.

SPIN-код: 2050-4004

Russian Federation

D. A. Berseneva

Sechenov First Moscow State Medical University Ministry of Health of Russian Federation

Email: berseneva1410@rambler.ru
ORCID iD: 0000-0001-5970-2240
Moscow Russian Federation

M. V. Balyasin

Sechenov First Moscow State Medical University Ministry of Health of Russian Federation

Email: max160203@gmail.com
ORCID iD: 0000-0002-3097-344X
Moscow Russian Federation

V. D. Parshin

Sechenov First Moscow State Medical University Ministry of Health of Russian Federation

Email: vdparshin@yandex.ru
Moscow Russian Federation

A. V. Lyundup

Sechenov First Moscow State Medical University Ministry of Health of Russian Federation

Email: lyundup@gmail.com
ORCID iD: 0000-0002-0102-5491

Кандидат медицинских наук, заведующий отделением клеточных технологий Института регенеративной медицины.

119991, Москва, ул. Трубецкая, д. 8, стр. 2, тел.: +7 (495) 609-14-00.

SPIN-код: 4954-3004

Russian Federation

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