Influence fatty acids on oxygen consumption by isolated rat cardiomyocytes in ischemic or diabetic heart disease

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Abstract

Background: one of the reasons of violation of the functional viability of the myocardium is considered to be the shortage oxygen and energy of the myocardium. Its reason is the inhibitory effect of fatty acids on glucose oxidation. Recently, however, new data have been published, proving the need for and importance of fatty acids in the maintenance and regulation of the functional activity of the myocardium in chronic pathology.

Objective: to investigate the influence of free polyunsaturated and saturated fatty acids (FA) on the oxygen uptake of isolated cardiomyocytes in intact rats and animals with ischemic or diabetic heart disease.

Methods: executed non-randomized controlled study. Considered 3 groups of male rats of Wistar line (weight 250-300 g) with 10 animals in each group. Myocardial infarction ("heart attack") caused by ligation of the left coronary artery, and diabetes ("diabetes") – intraperitoneal injection of streptozotocin. The group "control" was presented intact animals. Myocardial infarction caused by ligation of the left coronary artery, and diabetes by intraperitoneal injection of streptozotocin. Isolated cardiac myocytes were obtained by enzymatic method. Oxygen consumption was assessed polarographically at different saturation incubation medium with oxygen ([O2] £8 mg/l and ([O2] ≥16 mg/l).

Results: As the fatty acid used arachidonic and palmitic acid. It is established that the introduction to the incubation medium 20 μm arachidonic or palmitic fatty acid significantly increased the oxygen consumption of intact cardiomyocytes of rats. Both at the ischemic and at diabetic defeat of heart was obtained the opposite result. The most pronounced decrease of oxygen consumption are shown in diabetes mellitus.

Conclusion: Conclude that the inhibitory effect of LCD on the rate of oxygen consumption during the at the ischemic and at diabetic defeat of heart may be due to their influence on the barrier function of mitochondrial membranes of cardiomyocytes, the activity of membrane-associated enzymes and their associated processes.

About the authors

S. A. Afanasiev

Research Institute for Cardiology, Tomsk

Author for correspondence.
Email: tursky@cardio-tomsk.ru
Sergey A.Afanasiev, PhD, Professor, Head of Laboratory Russian Federation

M. V. Egorova

Research Institute for Cardiology, Tomsk

Email: mwegorova@yadex.ru
Margarita V. Egorova, PhD in Biology, Associate Professor Russian Federation

T. V. Kutsykova

Research Institute for Cardiology, Tomsk

Email: naf30@mail.ru
Tatyna V. Kutsykova, Post-graduate Student Russian Federation

S. V. Popov

Research Institute for Cardiology, Tomsk

Email: psv@cardio-tomsk.ru
Sergey V. Popov, Corresponding Member of RAS, Professor, PhD Russian Federation

References

  1. Оруджева С.А., Звягин А.А. Оценка функциональных резервов сердечно-сосудистой системы у больных сахарным диабетом. Опасности анестезии и возможности анестезиологического обеспечения больных с гнойно-некротическими формами синдрома диабетической стопы // Новости анестезиологии и реаниматологии. – 2006. – №3. – С. 1−19. [Orudzheva SA, Zvyagin AA. Otsenka funktsional’nykh rezervov serdechno-sosudistoi sistemy u bol’nykh sakharnym diabetom. Opasnosti anestezii i vozmozhnosti anesteziologicheskogo obespecheniya bol’nykh s gnoino-nekroticheskimi formami sindroma diabeticheskoi stopy. Novosti anesteziologii i reanimatologii. 2006;3:1−19. (In Russ).]
  2. Вельков В. Свободные жирные кислоты ― фактор риска инсулинорезистентности и ишемии: перспективы для оценки рисков и диагностики // Поликлиника. – 2008. – №4. – С. 50−51. [Vel’kov V. Svobodnye zhirnye kisloty ― novyi marker insulinorezistentnosti i ishemii: perspektivy dlya otsenki riskov i diagnostiki. Poliklinika. 2008;4:50−51. (In Russ).]
  3. Lopaschuk GD, Ussher JR, Folmes CD, et al. Myocardial fatty acid metabolism in health and disease. Physiol Rev. 2010;90(1):207−258. doi: 10.1152/physrev.00015.2009.
  4. Brindley DN, Kok BPC, Kienesberger PC, et al. Shedding light on the enigma of myocardial lipotoxicity: the involvement of known and putative regulators of fatty acid storage and mobilization. Am J Physiol. 2010;298:E897−E908. doi: 10.1152/ajpendo.00509.2009.
  5. Chowdhury R, Warnakula S, Kunutsor S, et al. Association of dietary, circulating, and supplement fatty acids with coronary risk: a systematic review and meta-analysis. Ann Intern Med. 2014;160(6):398−406. doi: 10.7326/M13-1788.
  6. Mozaffarian D, Lemaitre RH, King IB, et al. Plasma Phospholipid Long-Chain Omega-3 Fatty Acids and Total and Cause-Specific Mortality in Older Adults: the Cardiovascular Health Study. Ann Intern Med. 2013;158:515−525. doi: 10.7326/0003-4819-158-7-201304020-00003.
  7. Van Bilsen M, Planavila A. Fatty acids and cardiac disease: fuel carrying a messagе. Acta Physiol. 2014;211(3):476–490. doi: 10.1111/apha.12308.
  8. Реброва Т.Ю., Кондратьева Д.С., Афанасьев С.А., Барзах Е.И. Активность перекисного окисления липидов и функциональное состояние миокарда при ремоделировании сердца крыс после экспериментального инфаркта // Кардиология. – 2007. – №6. – С. 41−45. [Rebrova TY, Kondrat’eva DS, Afanas’ev SA, Barzakh EI. Activity of lipid peroxidation and functional state of the myocardium in remodeling of rat heart after experimental myocardial Infarction. Kardiologiya. 2007;6:41−45. (In Russ).]
  9. Афанасьев С.А., Реброва Т.Ю., Кондратьева Д.С. Особенности фосфолипидного состава мембран эритроцитов в условиях постинфарктного кардиосклероза // Биомедицинская химия. – 2007. – №53. – С. 541−546. [Afanas’ev SA, Rebrova TY, Kondrat’eva DS. Phospholipid composition of erythrocyte membranes under conditions of postmyocardial infarction cardiosclerosis. Biomeditsinskaya khimiya. 2007;53:541−546. (In Russ).]
  10. Афанасьев С.А., Кондратьева Д.С., Попов С.В. Разработка модели сочетанной патологии сердечной недостаточности и сахарного диабета 1-го типа в эксперименте // Бюллетень экспериментальной биологии и медицины. – 2012. – Т. 153. – №4. – С 523−526. [Afanasiev SA, Kondratyeva DS, Popov SV. Development of an Experimental Model of Cardiac Failure Combined with Type I Diabetes Mellitus. Bull Exp Biol Med. 2012;153(4):530-532.] doi: 10.1007/s10517-012-1759-8.
  11. Егорова М.В., Афанасьев С.А., Попов С.В. Простой метод выделения кардиомиоцитов из сердца взрослой крысы // Бюллетень экспериментальной биологии и медицины.– 2005. – Т. 140. – №9. – С. 357−360. [Egorova MV, Afanas’ev SA, Popov SV. A Simple Method for Isolation of Cardiomyocytes from Adult Rat Heart. Bull Exp Biol Med. 2005;140(3):370-373.] doi: 10.1007/s10517-005-0492-y.
  12. Егорова М.В., Афанасьев С.А., Попов С.В. Роль фосфолипазы А2 в активации дыхания изолированных кардиомиоцитов при постинфарктном кардиосклерозе // Бюллетень экспериментальной биологии и медицины. – 2008. – Т. 146. – №12. – С. 631−633. [Egorova MV, Afanas’ev SA, Popov SV. Role of Phospholipase A2 in Activation of Isolated Cardiomyocyte Respiration in Postinfarction Cardiosclerosis. Bull Exp Biol Med. 2009;146(6):695697.] doi: 10.1007/s10517-009-0398-1.
  13. Егорова М.В., Афанасьев С.А. Регуляторная роль свободных жирных кислот в поддержании мембранного гомеостаза митохондрий сердца при экспериментальной ишемии миокарда // Бюллетень сибирской медицины. – 2012. – №3. – С. 31−38. [Egorova MV, Afanas’ev SA. Regulatory role of free fatty acids in maintain of membrane homeostasis in heart mitochondria at experimental myocardial ischaemia. Byulleten’ sibirskoi meditsiny. 2012;3:31−38. (In Russ).]
  14. Александров А.А. Диабетическое сердце: схватка за митохондрии // Consilium medicum (Педиатрия). – 2003. – №5. – С. 509−513. [Aleksandrov AA. Diabeticheskoe serdtse: skhvatka za mitokhondrii. Consilium medicum (Pediatriya). 2003;5:509−513. (In Russ).]
  15. Ashrafian H, Redwood C, Blair E, et al. Hypertrophic cardiomyopathy: a paradigm for myocardial energy depletion. Trends Genet. 2003;19(5):263–268. doi: 10.1016/s0168-9525(03)00081-7.

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