THE EXPERIMENTAL MODEL OF AUTOIMMUNE PROCESS: THE ROLE OF EPIGENETIC VARIATION IN THE POPULATION OF MICE HYBRIDS
- Authors: Kolesnikova O.P.1, Kudaeva O.T.1, Vol'skii N.N.1, Goiman E.V.1, Gavrilova E.D.1, Perminova O.M.1, Demchenko E.N.1, Kozlov V.A.1
- Affiliations:
- Research Institute of Fundamental and Clinical Immunology, Novosibirsk, Russian Federation
- Issue: Vol 70, No 2 (2015)
- Pages: 152-158
- Section: PROCEEDINGS of the GENERAL MEETING of the Department of Medical Sciences, RAS
- URL: https://vestnikramn.spr-journal.ru/jour/article/view/50
- DOI: https://doi.org/10.15690/vramn.v70i2.1307
- Cite item
Abstract
Background: At the development of graft versus host disease in genetically homogeneous population of (C57Bl/6 x DBA/2) F1 mice two clinical phenotypes of SLE-like disease were revealed: lupus+ (immune complex glomerulonephritis and hemolytic anemia) and lupus – (hemolytic anemia). The GvHD phenotypic heterogeneity is determined by the Th1 /Th2-polarization: Th2 lymphocyte predominant activity, leads to the lupus+ development, or prevalence activity of Th1 cells, leads to the lupus– development. Objective: Our aim was to evaluate the possibility of using an experimental model of autoimmune disease for studying and testing of epigenetic modifications, shifting Th1 /Th2-
balance in vivo. Methods: Сhronic GVHD was induced in B6D2F1 mice by the transplantation of 130×10 6 parental DBA/2 splenocytes. Аnti-ds-DNA, total IgG and IgG1, IgG2а Abs were measured by ELISA. Results: Six- to 8-week-old female DBA/2 and B6D2F1 mice were obtained from Biological Research Laboratory (Novosibirsk). It was established that regular moderate physical activity (unladed swimming) shifted Th1 /Th2 balance towards Th1. This leads to a decrease in a population of recipients the lupus+ mice from 57 to 26% (p <0,001) with significantly reduced hypergammaglobulinemia (IgG from 2,8 to 2,0 mg/ml; p <0,047) and DNA antibodies titer from 0,18 to 0,12 OD (p =0,05). Administration of epigenetic modificator bisphenol A at low doses, which mimicking estrogen effects, enhances the proportion of lupus+ mice in experimental groups from 33 to 64% (p <0,001) and impairs their clinical status by the increasing the urine protein level from 2.8 to 4,2 mg/ml (p <0,001) in animals. Conclusion: Th1 /Th2 – balance presumably is determined by the immune system epigenetic modification in experimental mice, formed on the previous stages of ontogeny and defines the direction of immune processes development in individual animal.
About the authors
O. P. Kolesnikova
Research Institute of Fundamental and Clinical Immunology, Novosibirsk, Russian Federation
Author for correspondence.
Email: iscreen2001@mail.ru
Russian Federation
доктор медицинских наук, руководитель лаборатории экспериментальной иммуноте-
рапии НИИ фундаментальной и клинической иммунологии
Адрес: 630099, Новосибирск, ул. Ядринцевская, д. 14, тел.: +7 (383) 222-06-72
O. T. Kudaeva
Research Institute of Fundamental and Clinical Immunology, Novosibirsk, Russian Federation
Email: iscreen2001@mail.ru
Russian Federation
доктор биологических наук, ведущий научный сотрудник лаборатории эксперимен-
тальной иммунотерапии НИИ фундаментальной и клинической иммунологии
N. N. Vol'skii
Research Institute of Fundamental and Clinical Immunology, Novosibirsk, Russian Federation
Email: dtheory@yandex.ru
Russian Federation
кандидат медицинских наук, ведущий научный сотрудник лаборатории эксперимен-
тальной иммунотерапии НИИ фундаментальной и клинической иммунологии
Адрес: 630099, Новосибирск, ул. Ядринцевская, д. 14, тел.: +7 (383) 222-06-72
E. V. Goiman
Research Institute of Fundamental and Clinical Immunology, Novosibirsk, Russian Federation
Email: l.goiman@mail.ru
Russian Federation
кандидат медицинских наук, научный сотрудник лаборатории экспериментальной
иммунотерапии НИИ фундаментальной и клинической иммунологии
Адрес: 630099, Новосибирск, ул. Ядринцевская, д. 14, тел.: +7 (383) 222-06-72
E. D. Gavrilova
Research Institute of Fundamental and Clinical Immunology, Novosibirsk, Russian Federation
Email: edav.gavr@mail.ru
Russian Federation
кандидат биологических наук, старший научный сотрудник лаборатории эксперимен-
тальной иммунотерапии НИИ фундаментальной и клинической иммунологии
Адрес: 630099, Новосибирск, ул. Ядринцевская, д. 14, тел.: +7 (383) 222-06-72
O. M. Perminova
Research Institute of Fundamental and Clinical Immunology, Novosibirsk, Russian Federation
Email: pom.57@yandex.com
Russian Federation
кандидат медицинских наук, научный сотрудник лаборатории экспериментальной
иммунотерапии НИИ фундаментальной и клинической иммунологии
Адрес: 630099, Новосибирск, ул. Ядринцевская, д. 14, тел.: +7 (383) 222-06-72
E. N. Demchenko
Research Institute of Fundamental and Clinical Immunology, Novosibirsk, Russian Federation
Email: elena.demchenko@gmail.ru
Russian Federation
кандидат химических наук, научный сотрудник лаборатории экспериментальной
иммунотерапии НИИ фундаментальной и клинической иммунологии
Адрес: 630099, Новосибирск, ул. Ядринцевская, д. 14, тел.: +7 (383) 222-06-72
V. A. Kozlov
Research Institute of Fundamental and Clinical Immunology, Novosibirsk, Russian Federation
Email: vakoz40@yandex.ru
Russian Federation
доктор медицинских наук, профессор, академик РАН, директор НИИ фундамен-
тальной и клинической иммунологии
Адрес: 630099, Новосибирск, ул. Ядринцевская, д. 14, тел.: +7 (383) 222-26-74
References
- Eisenberg R., Via C. T cells, murine chronic graft-versus-host disease and autoimmunity. J. Autoimmun. 2012; 39: 240–247.
- Cher D., Mosmann T. Two types of murine helper T cell clone. II. Delayed-type hypersensitivity is mediated by TH1 clones. J. Immunol. 1987; 138: 3688–3694.
- Szyf M. Epigenetic therapeutics in autoimmune disease. Clin. Rev. Allerg. Immunol. 2010; 39 (1): 62–77.
- van Panhuys N., Le Gros G., McConnell J. Epigenetic regulation of Th2 cytokine expression in atopic diseases. Tissue Antigens. 2008; 72 (2): 91–97.
- Sanders V., Epigenetic regulation of Th1 and Th2 cell development. Brain Behav. Immun. 2006; 20: 317–324.
- Via C., Shearer G. T cell interactions in autoimmunity: insights from a murine model of graft-versus-host disease. Immunol. Today. 1988; 9: 207–213.
- 7. Anway M., Cupp A.S, Uzumcu M., Skinner M. Epigenetic transgenerational actions of endocrine disruptors and male fertility. Science. 2005; 308: 1466–1469.
- Kimura M., Gleichmann E. Depressed antibody responses to exogenous antigens in mice with lupus-like graft-versus-host disease. Clin. Immunol. Immunopathol. 1987; 43 (1): 97–109.
- Kolesnikova O.P., Kudaeva O.T., Loginov V.A., Robinson M.V., Kolesnikova S.M., Kozlov V.A. Indicators immunopoiesis erythro- and development of autoimmune diseases induced by chronic graft versus host disease. Vestnik AMN SSSR = Bulletin of AcademyMedical Sciences of the USSR. 1991;12:13–16.
- Gerlinskaya L.A., Moshkin M.P., Evsikov V.I. Methodical aspects of determining the level of stress in wild animals. Ekologiya = Ecology. 1993;1:97–100.
- Muracami M., Yoshioka H., Shirai T., Tsubata T., Honjo T. Prevention of autoimmune symptoms in autoimmune-prone mice by elimination of B-1 cells. Int. Immunol. 1995; 7 (5): 877–882.
- Nikolic B., Lee S., Bronson R.T, Grusby M., Sykes M. Th1 and Th2 mediate acute graft-versus-host disease, each with distinct end-organ targets. J. Clin. Invest. 2000; 105: 1289–1298.
- Morris S., Madden K., Adamovicz J., Gause W., Hubbard B., Gately M., Finkelman F. Effects of IL-12 on in vivo cytokine gene expression and Ig isotype selection. J. Immunol. 1994; 152: 1047–1056.
- Sukhenko T.G., Kolesnikova O.P., Filimonov P.N., Kozlov V.A. Status immuno- and erythropoiesis in mice with graft versus host disease in the immunocompromised. ZhMEI = Journal of microbiology, epidemiology and immunobiology. 1999; 4: 56–60.
- Reiss A.B., Awadallah N.W., Malhotra S., Montesinos M.C., Chan E.S., Javitt N.B., Cronstein B.N. Immune complexes and IFN-g decrease cholesterol 27-hydroxylase in human arterial endothelium and macrophages. J. Lipid Res. 2001; 42: 1913–1922.
- Chan R., Jiang P., Peng X., Tam L-S., Liao G., Li E. et al. Plasma DNA aberrations in systemic lupus erythematosus revealed by genomic and methylomic sequencing. Proc. Natl. Acad. Sci USA. 2014; 111 (49): 5302–5311.
- Whitacre C. Sex differences in autoimmune disease. Nat. Immunol. 2001; 2: 777–780.
- Waiserman A. Early-life exposure to endocrine disrupting chemicals and later-life health outcomes: an epigenetic bridge? Ading Dis. 2014; 5 (6): 419–429.
Statistics
Views
Abstract - 1312
PDF (Russian) - 289
Cited-By

Comments on this article
by smith sera (28.10.2019)
by Write My Essay (25.03.2020)
by Write My Essay (25.03.2020)
by Write My Essay (25.03.2020)
by Essay Writer (26.03.2020)
by Tom Banton (30.03.2020)
by Tom Banton (30.03.2020)
by Tom Banton (30.03.2020)
by Tom Banton (30.03.2020)
by Write Essay For Me (01.04.2020)
by Write Essay For Me (01.04.2020)
by mason lava lava (02.04.2020)
by Linda Helen (10.04.2020)
by Lily Jacob (15.04.2020)
by Lily Jacob (15.04.2020)
by Paul Anderson (18.04.2020)
by fiona basil (28.04.2020)
by fiona basil (28.04.2020)
by fiona basil (01.05.2020)
by fiona basil (01.05.2020)
by chris evans (05.05.2020)
by fiona basil (05.05.2020)
by fiona basil (05.05.2020)
by Maria Lena (07.05.2020)
by fiona basil (08.05.2020)
by fiona basil (08.05.2020)
by fiona basil (11.05.2020)
by Jack Ryan (11.05.2020)
by Robin Dunn (12.05.2020)
by fiona basil (13.05.2020)
by fiona basil (13.05.2020)
by fiona basil (14.05.2020)
by fiona basil (14.05.2020)
by Allie Aim (14.05.2020)
by fiona basil (15.05.2020)
by fiona basil (15.05.2020)
by Amanda Polanski (15.05.2020)
by fiona basil (16.05.2020)
by fiona basil (16.05.2020)
by Ricky Paul (18.05.2020)
by fiona basil (20.05.2020)
by John Collin (21.05.2020)
by John Collin (21.05.2020)
by John Collin (21.05.2020)
by Paul Anderson (21.05.2020)
by John Collin (24.05.2020)
by John Collin (24.05.2020)
by John Collin (24.05.2020)
by fiona basil (27.05.2020)
by Anerood Michel (03.06.2020)
by Anerood Michel (03.06.2020)
by fiona basil (04.06.2020)
by fiona basil (04.06.2020)
by fiona basil (08.06.2020)
by fiona basil (09.06.2020)
by King Alisah (09.06.2020)
by jack jons (11.06.2020)
by Dave Heller (11.06.2020)
by fiona basil (13.06.2020)
by fiona basil (13.06.2020)
by Paul Anderson (18.06.2020)
by Valeria Falerina (23.06.2020)
by afra jerald (25.06.2020)
by afra jerald (25.06.2020)
by afra jerald (27.06.2020)
by afra jerald (27.06.2020)
by afra jerald (29.06.2020)
by Robin Dunn (30.06.2020)
by Linda Driggers (30.06.2020)
by Emma Jackson (01.07.2020)
by afra jerald (09.07.2020)
by Aidan Connolly (10.07.2020)
by afra jerald (11.07.2020)
by afra jerald (11.07.2020)
by Andrew Williams (11.07.2020)
by afra jerald (13.07.2020)
by afra jerald (13.07.2020)
by afra jerald (13.07.2020)
by Andri Munandar (14.07.2020)
by afra jerald (14.07.2020)
by afra jerald (14.07.2020)
by afra jerald (15.07.2020)
by afra jerald (15.07.2020)
by Matthew Brick (15.07.2020)
by afra jerald (16.07.2020)
by afra jerald (16.07.2020)
by Benny Dyal (17.07.2020)
by Benny Dyal (17.07.2020)
by afra jerald (17.07.2020)
by afra jerald (17.07.2020)
by afra jerald (18.07.2020)
by afra jerald (18.07.2020)
by afra jerald (18.07.2020)
by afra jerald (23.07.2020)
by afra jerald (23.07.2020)
by afra jerald (24.07.2020)
by Evelyn Hermann (24.07.2020)
by Thamos Liam (30.07.2020)
by Robin Schneider (06.08.2020)
by Donald hardy (09.08.2020)
by Samuel Wellington (10.08.2020)
by drida infotech (11.08.2020)
by Lily Johnson (17.08.2020)
by angelina carlo (19.08.2020)
by angelina carlo (19.08.2020)
by angelina carlo (19.08.2020)
by Renee sinz (19.08.2020)
by angelina carlo (19.08.2020)
by angelina carlo (19.08.2020)
by jimmy martin (20.08.2020)
by shemarth watson (20.08.2020)
by Briar Guerrero (20.08.2020)
by jon win (31.08.2020)
by Viet bds (05.09.2020)
by afra jerald (07.09.2020)
by afra jerald (07.09.2020)
by afra jerald (09.09.2020)
by afra jerald (10.09.2020)
by afra jerald (10.09.2020)
by afra jerald (11.09.2020)
by afra jerald (11.09.2020)
by afra jerald (12.09.2020)
by afra jerald (12.09.2020)
by afra jerald (12.09.2020)
by afra jerald (14.09.2020)
by afra jerald (14.09.2020)
by afra jerald (15.09.2020)
by afra jerald (15.09.2020)
by afra jerald (15.09.2020)
by afra jerald (16.09.2020)
by afra jerald (16.09.2020)
by afra jerald (16.09.2020)
by afra jerald (17.09.2020)
by afra jerald (17.09.2020)
by afra jerald (17.09.2020)
by Hanna Swarn (17.09.2020)
by afra jerald (18.09.2020)
by afra jerald (22.09.2020)
by Lucy Miranda (25.09.2020)
by afra jerald (25.09.2020)
by afra jerald (25.09.2020)
by afra jerald (26.09.2020)
by afra jerald (26.09.2020)
by afra jerald (28.09.2020)
by afra jerald (30.09.2020)
by afra jerald (01.10.2020)
by afra jerald (02.10.2020)
by fiona basil (06.10.2020)
by sherly sylvia (06.10.2020)
by Chris John (06.10.2020)
by andrew symond symond (08.10.2020)
by sherly sylvia (08.10.2020)
by sherly sylvia (09.10.2020)
by sherly sylvia (09.10.2020)
by jack jons (09.10.2020)
by anthony Maosn (09.10.2020)
by anthony Maosn (09.10.2020)
by afra jerald (10.10.2020)
by afra jerald (10.10.2020)
by Jade Wyatt (12.10.2020)
by sherly sylvia (13.10.2020)
by sherly sylvia (13.10.2020)
by afra jerald (14.10.2020)
by sherly sylvia (14.10.2020)
by sherly sylvia (14.10.2020)
by Clairei ller (14.10.2020)
by sherly sylvia (15.10.2020)
by sherly sylvia (15.10.2020)
by Nicole Nelson (15.10.2020)
by Scott Short (15.10.2020)
by Jacob Joyce (15.10.2020)
by Faith Marsden (15.10.2020)
by afra jerald (16.10.2020)
by sherly sylvia (16.10.2020)
by sherly sylvia (16.10.2020)
by Byrann Mckinley (16.10.2020)
by Wilton Bonner (16.10.2020)
by Armando Mulroney (16.10.2020)
by fiona basil (17.10.2020)
by sherly sylvia (17.10.2020)
by malina leoo (17.10.2020)
by fiona basil (19.10.2020)
by sherly sylvia (19.10.2020)
by Jason Boo (19.10.2020)
by tom lewis (19.10.2020)
by fiona basil (20.10.2020)
by sherly sylvia (20.10.2020)
by fiona basil (21.10.2020)
by sherly sylvia (21.10.2020)
by Samuel Dominic (22.10.2020)
by fiona basil (23.10.2020)
by fiona basil (23.10.2020)
by sherly sylvia (23.10.2020)
by Harry Justin (23.10.2020)
by fiona basil (24.10.2020)
by sherly sylvia (24.10.2020)
by sherly sylvia (26.10.2020)
by fiona basil (26.10.2020)
by Jason Boo (26.10.2020)
by Jason Boo (26.10.2020)
by fiona basil (28.10.2020)
by sherly sylvia (28.10.2020)
by sherly sylvia (29.10.2020)
by fiona basil (31.10.2020)
by fiona basil (31.10.2020)
by sherly sylvia (31.10.2020)
by sherly sylvia (31.10.2020)
by fiona basil (02.11.2020)
by fiona basil (02.11.2020)
by sherly sylvia (02.11.2020)
by sherly sylvia (02.11.2020)
by fiona basil (03.11.2020)
by fiona basil (03.11.2020)
by sherly sylvia (03.11.2020)
by sherly sylvia (03.11.2020)
by sherly sylvia (04.11.2020)
by fiona basil (04.11.2020)
by fiona basil (05.11.2020)
by sherly sylvia (05.11.2020)
by Akash Mishra (05.11.2020)
by sherly sylvia (06.11.2020)
by fiona basil (06.11.2020)
by fiona basil (06.11.2020)
by sherly sylvia (07.11.2020)
by fiona basil (07.11.2020)
by sherly sylvia (07.11.2020)
by fiona basil (07.11.2020)
by William Smith (07.11.2020)
by Chris Kyle (07.11.2020)
by Chris Kyle (07.11.2020)
by sherly sylvia (09.11.2020)
by fiona basil (09.11.2020)
by sherly sylvia (10.11.2020)
by sherly sylvia (10.11.2020)
by fiona basil (10.11.2020)
by fiona basil (10.11.2020)
by Steve Finch (10.11.2020)
by sherly sylvia (11.11.2020)
by fiona basil (11.11.2020)
by sherly sylvia (11.11.2020)
by fiona basil (11.11.2020)
by john fahad (11.11.2020)
by fiona basil (12.11.2020)
by sherly sylvia (12.11.2020)
by Jason Smith (12.11.2020)
by fiona basil (13.11.2020)
by sherly sylvia (13.11.2020)
by fiona basil (16.11.2020)
by sherly sylvia (16.11.2020)
by fiona basil (16.11.2020)
by sherly sylvia (16.11.2020)
by Polly Frazier (16.11.2020)
by Brian Stauffer (16.11.2020)
by fiona basil (17.11.2020)
by fiona basil (17.11.2020)
by sherly sylvia (17.11.2020)
by sherly sylvia (17.11.2020)
by fiona basil (18.11.2020)
by sherly sylvia (18.11.2020)
by sherly sylvia (19.11.2020)
by valera salata (19.11.2020)
by Alicia Pit (20.11.2020)
by fiona basil (20.11.2020)
by sherly sylvia (20.11.2020)
by fiona basil (21.11.2020)
by sherly sylvia (21.11.2020)
by fiona basil (23.11.2020)
by fiona basil (23.11.2020)
by sherly sylvia (23.11.2020)
by sherly sylvia (23.11.2020)
by smart access (23.11.2020)
by Dalton James (24.11.2020)
by fiona basil (24.11.2020)
by Doris Hall (24.11.2020)
by Molska Orsesen (26.11.2020)
by Molska Orsesen (26.11.2020)
by sherly sylvia (27.11.2020)
by fiona basil (27.11.2020)
by fiona basil (27.11.2020)
by fiona basil (28.11.2020)
by fiona basil (28.11.2020)
by sherly sylvia (28.11.2020)
by sherly sylvia (28.11.2020)
by sherly sylvia (30.11.2020)
by fiona basil (30.11.2020)
by Jade Wyatt (30.11.2020)
by sherly sylvia (04.12.2020)
by sherly sylvia (04.12.2020)
by sherly sylvia (05.12.2020)
by sherly sylvia (05.12.2020)
by sherly sylvia (07.12.2020)
by sherly sylvia (07.12.2020)
by sherly sylvia (08.12.2020)
by anna alison (08.12.2020)
by Alex Alison (08.12.2020)
by Andy Anderson (08.12.2020)
by Jason Smith (09.12.2020)
by Jason Smith (09.12.2020)
by Jason Smith (10.12.2020)
by sherly sylvia (10.12.2020)
by sherly sylvia (14.12.2020)
by sherly sylvia (15.12.2020)
by sherly sylvia (16.12.2020)
by fiona basil (16.12.2020)
by sherly sylvia (17.12.2020)
by fiona basil (17.12.2020)
by fiona basil (17.12.2020)
by sherly sylvia (18.12.2020)
by fiona basil (18.12.2020)
by Steve Finch (18.12.2020)
by sherly sylvia (19.12.2020)
by fiona basil (19.12.2020)
by sherly sylvia (21.12.2020)
by fiona basil (21.12.2020)
by fiona basil (22.12.2020)
by fiona basil (22.12.2020)
by sherly sylvia (22.12.2020)
by sherly sylvia (23.12.2020)
by sherly sylvia (23.12.2020)
by fiona basil (23.12.2020)
by fiona basil (23.12.2020)
by sherly sylvia (31.12.2020)
by fiona basil (31.12.2020)
by fiona basil (31.12.2020)
by Malena Morgan (31.12.2020)
by sherly sylvia (02.01.2021)
by fiona basil (02.01.2021)
by fiona basil (02.01.2021)
by sherly sylvia (04.01.2021)
by sherly sylvia (05.01.2021)
by fiona basil (05.01.2021)
by Lola Nickson (05.01.2021)
by Shaurya Dickson (05.01.2021)
by fiona basil (07.01.2021)
by sherly sylvia (07.01.2021)
by fiona basil (07.01.2021)
by Essay UK (07.01.2021)
by fiona basil (09.01.2021)
by fiona basil (09.01.2021)
by sherly sylvia (09.01.2021)
by sherly sylvia (09.01.2021)
by sherly sylvia (11.01.2021)
by sherly sylvia (11.01.2021)
by fiona basil (11.01.2021)
by fiona basil (11.01.2021)
by fiona basil (12.01.2021)
by fiona basil (12.01.2021)
by sherly sylvia (12.01.2021)
by sherly sylvia (12.01.2021)
by sherly sylvia (13.01.2021)
by fiona basil (13.01.2021)
by sherly sylvia (13.01.2021)
by fiona basil (13.01.2021)
by sherly sylvia (13.01.2021)
by fiona basil (13.01.2021)
by jack jons (14.01.2021)
by James William (15.01.2021)
by fiona basil (16.01.2021)
by sherly sylvia (16.01.2021)
by sherly sylvia (16.01.2021)
by fiona basil (16.01.2021)
by John F Taller (17.01.2021)
by sherly sylvia (18.01.2021)
by fiona basil (18.01.2021)
by John Perry (19.01.2021)
by iphone tamiri tamiri (19.01.2021)
by Jacket Arena (20.01.2021)
by ariel wilson (20.01.2021)
by The Leather Makers (20.01.2021)
by daniel craig (21.01.2021)
by Ronan Kevin (22.01.2021)
by Sheridan Lee (23.01.2021)
by Phill Wagner (24.01.2021)
by Phill Wagner (24.01.2021)
by Alexey Alex (24.01.2021)
by sherly sylvia (25.01.2021)
by fiona basil (25.01.2021)
by Mark Ellis (25.01.2021)
by Mark Ellis (25.01.2021)
by Mark Ellis (25.01.2021)