MICROEVOLUTION OF CHOLERA AGENT IN THE MODERN PERIOD

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Abstract

Aim: To carry out comparative molecular genetic analysis of highly pathogenic atypical Vibrio cholerae strains biovar El Tor, isolated in the territory of RF, in order to determine micro-evolutionary alterations of cholera agent in the modern period. Materials and methods: 38 clinical strains have been examined by means of polymerase chain reaction, sequencing and MLVA-analysis. The selected strains were isolated at different periods of time during cholera epidemic complications and differed between each other in virulence. Results: It is demonstrated that new variants have emerged in the course of short-term microevolution. Their genome structure and function differ from those of all previously known strains. The genome alterations have been caused by point mutations in ctxB и tcpA genes associated with virulence and located in CTXφ prophage and pathogenicity island VPI-1 respectively, as well as by the extended deletion in pandemicity island VSP-II. Presented is the dynamics of genome structure and function alterations in modern strains. Conclusion: The discovered genomic alterations in the new variants of the agent evolved in the process of microevolution are indicative of their epidemic potential enhancement and probability of virulence potentiation.

About the authors

N. I. Smirnova

Russian Research Anti-Plague Institute «Microbe»

Author for correspondence.
Email: rusrapi@microbe.ru

PhD, professor, Head of the Microbiology Department of the Russian Research Anti-Plague Institute «Microbe»

Russian Federation

D. A. Agafonov

Russian Research Anti-Plague Institute «Microbe», Saratov, Russian Federation

Email: rusrapi@microbe.ru
аспирант лаборатории патогенных вибрионов Российского научно-исследователь- ского противочумного института «Микроб» Адрес: 410005, Саратов, ул. Университетская, д. 46, тел: +7 (8452) 26-47-23, Russian Federation

T. A. Kul'shan'

Russian Research Anti-Plague Institute «Microbe», Saratov, Russian Federation

Email: rusrapi@microbe.ru
кандидат медицинских наук, научный сотрудник лаборатории патогенных вибрионов Российского научно-исследовательского противочумного института «Микроб» Адрес: 410005, Саратов, ул. Университетская, д. 46, тел: +7 (8452) 26-47-23 Russian Federation

Ya. M. Krasnov

Russian Research Anti-Plague Institute «Microbe», Saratov, Russian Federation

Email: rusrapi@microbe.ru
кандидат химических наук, заведующий лабораторией геномного и протеомного анализа Российского научно-исследовательского противочумного института «Микроб» Адрес: 410005, Саратов, ул. Университетская, д. 46, тел: +7 (8452) 26-47-23 Russian Federation

V. V. Kutyrev

Russian Research Anti-Plague Institute «Microbe», Saratov, Russian Federation

Email: rusrapi@microbe.ru
доктор медицинских наук, профессор, академик РАМН, директор Российского научно-исследовательского противочумного института «Микроб» Адрес: 410005, Саратов, ул. Университетская, д. 46, тел: +7 (8452) 26-21-31 Russian Federation

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