CALF BLOOD GEMODIALIZAT REDUCES NEUROTOXIC EFFECT OF HYDROGEN PEROXIDE ON HUMAN NEUROBLASTOMA CELLS

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Abstract

Objective: Our aim was to study the effect of calf blood gemodializat  on apoptosis and intracellular signaling pathways of neuroblastoma cells SK-N-SH human. Methods: Apoptosis was recorded by fluorescent microscopy using Hoechst 33342. Necrosis cells was monitored by propidium iodide. The fluorescence of the cells was recorded on a fluorescence inverted microscope Keyence BZ8100 (Japan). Formation of reactive oxygen species (ROS) in the cells of SK-N-SH was determined using nitroblue tetrazolium by absorbance at 620 nm on a plate reader «Uniplan». Results: When adding hydrogen peroxide to the background of the calf blood gemodializat been decreasing apoptosis of these cells with 43 to 17% relative to apoptosis in the presence of a hydrogen peroxide. Under these conditions, the calf blood gemodializat  significantly reduced ROS formation in human neuroblastoma cells SK-N-SH by the action of hydrogen peroxide. In these cells, we investigated the influence of calf blood gemodializat  on apoptosis and intracellular signaling pathway involving mitogen-activated protein kinase (p38MAPK), extracellular regulatory kinase (ERK), phosphatidylinositol 3-kinase (PI-3K) and-Jun-N-terminal kinase (JNK) using their selective inhibitors. Conclusion: It was shown that the mechanism of the protective effect of calf blood gemodializat   against peroxide-induced apoptosis in SK-N-SH dominant role is played by p38 MAPK and PI-3K.

 

About the authors

M. M. Yurinskaya

Sechenov First Moscow State Medical University, Moscow, Russian Federation
Institute of Cell Biophysics, Moscow Region, Russian Federation

Author for correspondence.
Email: marinayurin@mail.ru
кандидат биологических наук, старший научный сотрудник лаборатории экстре- мальных состояний НИЦ Первого МГМУ им. И.М. Сеченова, ведущий научный сотрудник Института биофизики клетки РАН Адрес: 142290, Московская обл., Пущино, ул.Институтская, д. 3, тел.: +7 (4967) 73-26-83 Russian Federation

M. G. Vinokurov

Sechenov First Moscow State Medical University, Moscow, Russian Federation
Institute of Cell Biophysics, Moscow Region, Russian Federation

Email: mgvinokurov@rambler.ru
доктор биологических наук, главный научный сотрудник лаборатории экстре- мальных состояний НИЦ Первого МГМУ им. И.М. Сеченова, заведующий лабораторией регуляции апоптоза Института биофизики клетки РАН Адрес: 142290, Московская обл., Пущино, ул.Институтская, д. 3, тел.: +7 (4967) 73-26-83 Russian Federation

E. I. Astashkin

Sechenov First Moscow State Medical University, Moscow, Russian Federation

Email: 287ast@mail.ru
доктор биологических наук, профессор кафедры патологии, заведующий лабораторией экстремальных состояний НИЦ Первого МГМУ им. И.М. Сеченова Адрес: 119992, Москва, ул. Трубецкая д. 8, стр. 1, тел.: +7 (495) 622-96-01

S. V. Grachev

Sechenov First Moscow State Medical University, Moscow, Russian Federation

Email: grachevscience@gmail.com
доктор медицинских наук, академик РАН, заведующий кафедрой патологии Первого МГМУ им. И.М. Сеченова Адрес: 119992, Москва, ул. Трубецкая д. 8, стр. 1, тел.: +7 (499) 248-31-22

N. S. Orekhova

Sechenov First Moscow State Medical University, Moscow, Russian Federation

Email: 287ast@mail.ru
кандидат медицинских наук, старший научный сотрудник лаборатории экстре- мальных состояний НИЦ Первого МГМУ им. И.М. Сеченова Адрес: 119992, Москва, ул. Трубецкая д. 8, стр. 1, тел.: +7 (495) 622-96-01 Russian Federation

A. N. Novikova

Sechenov First Moscow State Medical University, Moscow, Russian Federation

Email: 287ast@mail.ru
лаборант-исследователь лаборатории экстремальных состояний НИЦ Первого МГМУ им. И.М. Сеченова Адрес:119992, Москва, ул. Трубецкая д. 8, стр. 1, тел.: +7 (495) 622-96-01 Russian Federation

I. N. Sokolova

Sechenov First Moscow State Medical University, Moscow, Russian Federation

Email: 287ast@mail.ru
кандидат медицинских наук, ведущий научный сотрудник лаборатории функцио- нальных методов исследования и рациональной фармакотерапии сердечно-сосудистых заболеваний НИЦ Первого МГМУ им. И.М. Сеченова Адрес: 119992, Москва, ул. Трубецкая д. 8, стр. 1, тел.: +7 (499) 972-96-12 Russian Federation

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