EXPERIMENTAL MODEL OF OCULAR ISСHEMIC DISEASES

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Abstract

The review presents the most common methods of modeling of retinal ischemia in vitro (chemical ischemia with iodoacetic acid, incubation of the retinal pigment epithelium cells with oligomycin, deprivation of oxygen and glucose) and in vivo (a model with increased intraocular pressure, cerebral artery occlusion, chronic ligation of the carotid arteries, photocoagulation of the retinal vessels, occlusion of the central retinal artery, endothelin-1 administration). Modeling ischemic injury in rats is the most frequently used method in studies, because the blood supply of their eyes is similar to blood flow in the human eyes. Each method has its own advantages and disadvantages. Application of methods depends on the purpose of the experimental study. Currently model of ocular ischemic disease can be obtained easily by injecting vasoconstrictive drug endothelin-1. It is the most widely used method of high intraocular pressure induced ocular ischemic damage similar to glaucoma, occlusion of central retinal artery or ophthalmic artery in human. The development of experimental models of ocular ischemic diseases and detailed investigation of mechanisms of impairment of microcirculation are useful for improve the efficiency of diagnostic and treatment of ischemic diseases of retina and optic nerve.

 

About the authors

T. N. Kiseleva

Helmholtz Moscow Research Institute of Eye Diseases, Russian Federation

Author for correspondence.
Email: tkisseleva@yandex.ru
доктор медицинских наук, профессор, руководитель отделения ультразвука Москов- ского НИИ глазных болезней им. Гельмгольца Адрес: 105062, Москва, ул. Садовая-Черногрязская, д. 14/19, тел.: +7 (495) 624-31-34 Russian Federation

A. V. Chudin

Helmholtz Moscow Research Institute of Eye Diseases, Russian Federation

Email: ru27@mail.ru
аспирант отдела ультразвука Московского НИИ глазных болезней им. Гельмгольца Адрес: 105062, Москва, ул. Садовая-Черногрязская, д. 14/19, тел.: +7 (495) 624-31-34 Russian Federation

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