THE INFLUENCE OF POINT MUTATIONS IN THE EPSTEIN-BARR VIRUS LMP1 ONCOGENE ON THE CELL CYTOSKELETON AND ACTIVATION OF INDUCIBLE FORM OF NO SYNTHASE

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Abstract


One of the latent proteins encoded by the Epstein−Barr virus (EBV), the latent membrane protein 1 (LMP1), plays a key role in developing of EBV-associated human malignancies. Polymorphism of LMP1 protein is its characteristic feature. Some specific mutations in LMP1 genome have previously been detected in different geographic regions, however, the influence of these mutations on functional activity of LMP1 was not still determined. In this study we demonstrated for the first time the significance of individual point mutations among common ones observed in LMP1 and their combination on activation of the inducible form of nitric oxide synthase (iNOS). In addition, the influence of above mutations localized in the CTAR regions of the LMP1 molecule has also been investigated on structural components of the fibroblasts of the Rat1cell line.

 


About the authors

S. V. Diduk

N.N. Blokhin Russian Cancer Research Center RAMS

Author for correspondence.
Email: diduksv@rambler.ru

Russian Federation кандидат биологических наук, научный сотрудник лаборатории вирусного канцерогенеза НИИ канцерогенеза Российского онкологического научного центра имени Н.Н. Блохина РАМН Адрес: 115478, Москва, Каширское шоссе, д. 24 Тел.: (495) 323-57-55, факс: (495) 324-12-05

K. V. Smirnova

N.N. Blokhin Russian Cancer Research Center RAMS

Email: smirnovakv@rambler.ru

Russian Federation кандидат биологических наук, научный сотрудник лаборатории вирусного канцероге- неза НИИ канцерогенеза Российского онкологического научного центра имени Н.Н. Блохина РАМН Адрес: 115478, Москва, Каширское шоссе, д. 24

V. E. Gurtsevitch

N.N. Blokhin Russian Cancer Research Center RAMS

Email: gurtsevitch@yahoo.com

Russian Federation доктор медицинских наук, профессор, заведующий лабораторией вирусного канце- рогенеза НИИ канцерогенеза Российского онкологического научного центра имени Н.Н. Блохина РАМН Адрес: 115478, Москва, Каширское шоссе, д. 24 Тел.: (495) 324-25-64

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