INFLUENCE OF REGULATORY T CELLS ON THE FUNCTIONING OF NATURAL KILLER CELLS DURING CANCER IMMUNOTHERAPY

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Abstract


One of the common arguments against cancer immunotherapy based on natural killer (NK) cells activated in the presence of interleukin-2 (IL-2) is the probability of the activation of regulatory T cells (Tregs) by IL-2 besides NK cells. Thus, we have monitored numbers of FoxP3+CD4+CD25+ T cells in the samples of healthy volunteers’ peripheral blood mononuclear cells (PBMCs) cultured with or without IL-2. We observed marked increase in the percentages of the CD4+CD25+ T cells in the presence of IL-2. Proportions of Foxp3+CD4+CD25+ T cells feebly increased, remained on the same level or even decreased compared to PBMCs cultured without exogenous IL-2. Based on the absence of FoxP3 expression, most of the CD4+CD25+ T cells purified from IL-2 activated PBMCs were not Tregs, but activated Th cells. Moreover, the addition of the purified supposed Tregs to samples of activated NK cells never inhibited their cytotoxic reactions.

 


About the authors

I. O. Chikileva

Establishment of the Russian Academy for Medical Sciences N.N. Blokhin Russian Cancer Research Center RAMS

Author for correspondence.
Email: irinatchikileva@mail.ru

Russian Federation кандидат биологических наук, младший научный сотрудник лаборатории клеточного иммунитета НИИ ЭДиТО ФГБУ «Российский онкологический научный центр им. Н.Н. Блохина» РАМН Адрес: 115478, Москва, Каширское ш., д. 2 Тел./факс: 8(495) 324-27-94

I. Zh. Shubina

Establishment of the Russian Academy for Medical Sciences N.N. Blokhin Russian Cancer Research Center RAMS

Email: irinashubina@mail.ru

Russian Federation кандидат биологических наук, старший научный сотрудник лаборатории клеточного иммунитета НИИ ЭДиТО ФГБУ «Российский онкологический научный центр им. Н.Н. Блохина» РАМН Адрес: 115478, Москва, Каширское ш., д. 24 Тел./факс: 8(495) 324-27-94

E. V. Kiselevskii

Establishment of the Russian Academy for Medical Sciences N.N. Blokhin Russian Cancer Research Center RAMS

Email: kisele@inbox.ru

Russian Federation доктор медицинских наук, профессор, заведующий лабораторией клеточного иммунитета НИИ ЭДиТО ФГБУ «Российский онкологический научный центр им. Н.Н. Блохина» РАМН Адрес: 115478, Москва, Каширское ш., д. 24 Тел./факс: 8(495)324-27-94

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