EVALUATION OF ASSOCIATION BETWEEN 9 GENETIC POLYMORPHISM AND MYOCARDIAL INFARCTION IN THE SIBERIAN POPULATION

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Abstract

Aim: to evaluate association between genetic polymorphism (SNPs) and myocardial infarction (identified in recent GWAS) as markers of high risk of myocardial infarction (MI) in Siberian population. Patients were divided into 2 groups — MI patients and control group (ratio 1:2) and presented the sapmle of population of Novosibirsk (9400 patients, 45–69 years) within international project HAPIEE (Health, Alcohol and Psychosocial factors In Eastern Europe). 200 patients with MI (129 men, 71 women) were included. Control group — individuals without MI (420) matched for age and sex. Genomic DNA was extracted from venous blood by phenol-chloroform extraction. Gene polymorphism of genes tested by real-time PCR according to protocol (probes TaqMan, Applied Biosystems, USA) with the use of ABI 7900HT. The following SNPs were studied: rs28711149, rs499818, rs619203, rs10757278 and rs1333049 (hr. 9), rs1376251, rs2549513, rs4804611, rs17465637. The association of SNP and MI was confirmed for 4 of 9 studied SNPs: rs1333049 (hr. 9), rs10757278 (hr. 9), rs499818 (hr. 6), rs619203 gene ROS1. Heart rate was associated with rs1333049 and rs10757278. Glucose level was associated with rs619203, rs28711149 and rs1376251. Total cholesterol and atherogenic index was associated with rs28711149. For the first time in Russian population the associations of GWAS with myocardial infarction SNPs was detected for rs619203, rs499818, rs1333049 and rs10757278. These genetic markers can be used for assessing the risk of myocardial infarction in Russian population.

 

About the authors

V. N. Maximov

Institute of Internal medicine SB RAMS, Novosibirsk
Novosibirsk State Medical University, department of medical genetics

Author for correspondence.
Email: medik11@mail.ru
доктор медицинских наук, внештатный научный сотрудник лаборатории молекулярно-генетических исследований терапевтических заболеваний НИИ терапии Сибирского отделения РАМН Адрес: 630089, Новосибирск, ул. Б. Богаткова, д. 175/1 Тел./факс: (383) 264-25-16 Russian Federation

I. V. Kulikov

Institute of Internal medicine SB RAMS, Novosibirsk
Institute of Cytology and Genetics SD RAS, Novosibirsk

Email: 248945@mail.ru
кандидат медицинских наук, старший научный сотрудник лаборатории молекулярно- генетических исследований терапевтических заболеваний НИИ терапии Сибирского отделения РАМН Адрес: 630089, Новосибирск, ул. Б. Богаткова, д. 175/1 Тел./факс: (383) 264-25-16 Russian Federation

P. S. Orlov

Institute of Cytology and Genetics SD RAS, Novosibirsk

Email: orlovpavel86@gmail.com
аспирант Института цитологии и генетики Сибирского отделения РАН Адрес: 630090, Новосибирск, пр-т Лаврентьева, д. 10 Russian Federation

V. V. Gafarov

Institute of Internal medicine SB RAMS, Novosibirsk

Email: valery.gafarov@gmail.com
доктор медицинских наук, профессор, заведующий лабораторией психологических и социологических проблем терапевтических заболеваний НИИ терапии Сибирского отделения РАМН Адрес: 630089, Новосибирск, ул. Б. Богаткова, д. 175/1 Тел./факс: (383) 264-25-16 Russian Federation

S. K. Malyutina

Institute of Internal medicine SB RAMS, Novosibirsk
Novosibirsk State Medical University, department of medical genetics

Email: smalyutina@hotmail.com
доктор медицинских наук, профессор, руководитель группы неинвазивной диа- гностики лаборатории этиопатогенеза и клиники внутренних заболеваний НИИ терапии Сибирского отделения РАМН Адрес: 630089, Новосибирск, ул. Б. Богаткова, д. 175/1 Тел./факс: (383) 264-25-16 Russian Federation

A. G. Romashchenko

Institute of Cytology and Genetics SD RAS, Novosibirsk

Email: romasch@bionet.nsc.ru
кандидат биологических наук, заведующая лабораторией молекулярных основ генетики животных Института цитологии и генетики Сибирского отделения РАН Адрес: 630090, Новосибирск, пр-т Лаврентьева, д. 10 Тел: (383) 363-49-74 Russian Federation

M. I. Voevoda

Institute of Internal medicine SB RAMS, Novosibirsk
Institute of Cytology and Genetics SD RAS, Novosibirsk

Email: mvoevoda@ya.ru
доктор медицинских наук, профессор, член-корреспондент РАМН, директор НИИ терапии Сибирского отделения РАМН Адрес: 630089, Новосибирск, ул. Б. Богаткова, д. 175/1 Тел./факс: (383) 264-25-16 Russian Federation

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