GLUTATHION AS AN IMPORTANT COMPONENT OF THIOSULFID SYSTEM OF INFERTILITY PATHOGENESIS IN OVERWEIGHT MEN

Cover Page

Abstract


Aim: to study the glutathionic part of thiosulfid system and oxidative stress in infertile men with different body mass index. Patients and methods: examined 60 infertile men with normal and overweight, and 40 healthy men with proven fertility. Active components of the antioxidant defense system of the body was assessed by the level of reduced and oxidized glutathione by the method of PY Hissin, determination of TBA-active products was carried out by the method VB Gavrilova. In the analysis of inter-group differences for independent samples was used parametric Student criterion. Results: the oxidative stress in obese men was characterized by significant elevation of oxidized form of glutathione (GSSG) in comparison with its reduced form. We discussed our results in the context of GSSG «quantivalence» to numerous of cellular processes in the setting of metabolic disorders in infertile and obese men. The ratio of reduced glutathione to oxidized infertile men decreased by 1,5 times in patients with obesity and 1,2 times in those with normal body mass index. Conclusions: Glutathione is the most important link in the study of oxidative stress in men with infertility. Raising the level of oxidized glutathione is due to the presence of his additional functions that are implemented at higher values of body mass index, especially in association with infertility.

 


About the authors

L. I. Kolesnikova

Scientific Center of the Problems of Family Health and Human Reproduction, Irkutsk, Russian Federation

Author for correspondence.
Email: iphr@sbamsr.irk.ru

Russian Federation

PhD, Professor, RAMS academician, Director of the Scientific Center of Family Health and Human Reproduction Problems, Siberian Branch of Russian Academy of Medical Sciences
Address: 664003, Irkutsk, Timiryazeva Street, 16; tel.: (3952) 20-76-36, 20-73-67

O. A. Vanteeva

Scientific Center of the Problems of Family Health and Human Reproduction, Irkutsk, Russian Federation

Email: olga_tolpygina@mail.ru

Russian Federation PhD student, Scientific Center of Family Health and Human Reproduction Problems, Siberian Branch of Russian Academy of Medical Sciences
Address: 664003, Irkutsk, Timiryazeva Street, 16; tel.: (3952) 20-76-36

N. A. Kurashova

Scientific Center of the Problems of Family Health and Human Reproduction, Irkutsk, Russian Federation

Email: nakurashova@yandex.ru

Russian Federation

PhD, Research Worker, Scientific Center of Family Health and Human Reproduction Problems, Siberian Branch of Russian Academy of Medical Sciences
Address: 664003, Irkutsk, Timiryazeva Street, 16; tel.: (3952) 20-76-36

B. Ya. Vlasov

Scientific Center of the Problems of Family Health and Human Reproduction, Irkutsk, Russian Federation

Email: iphr@sbamsr.irk.ru

Russian Federation

PhD, Professor, Senior Research Worker, Laboratory of Physiology and Pathology of endocrine system, Scientific Center of Family Health and Human Reproduction Problems, Siberian Branch of Russian Academy of Medical Sciences
Address: 664003, Irkutsk, Timiryazeva Street, 16; tel.: (3952) 20-76-36, 20-73-67

References

  1. Kulakov V.I. Besplodnyi brak. Sovremennye podkhody k diagnostike i lecheniyu. [Infertile couple. Current approaches to diagnosis and treatment] Мoscow: GEOTAR.-Media. 2005. 616 p.
  2. Sukhikh G.T., Bozhedomov V.A. Muzhskoe besplodie [Male Infertility]. Мoscow: Eksmo. 2009. 240 p.
  3. Mel'nichenko G.A., Romantsova T.I. Ozhirenie [Obesity]. Pod red. I.I. Dedova, G.A. Mel'nichenko [I.I. Dedov, G.A. Mel'nichenko (editors)]. Мoscow: Meditsinskoe informatsionnoe agenstvo. 2004. 16–43 p.
  4. Sallmen M., Sandler D., Hoppin J.A., Blair A., Baird D.D. Reduced fertility among overweight and obese men. Epidemiology. 2006; 17 (5): 520–523.
  5. Fisher-Wellman K.H., Bloomer R.J. Exacerbated postprandial oxidative stress induced by the acute intake of a lipid meal compared to isoenergetically administered carbohydrate, protein, and mixed meals in young, healthy men. J. Am. Coll. Nutr. 2010; 29: 373–381.
  6. Bardymova T.P., Ukhova A.Yu., Darenskaya M.A. Lipid peroxidation and antioxidant protection in patients with diabetes mellitus. Sibirskii med. zhurn. (Irkutsk)- The Siberian Medical Journal (Irkutsk).2010; 6: 106–108.
  7. Kolesnikova L.I., Darenskaya M.A., Grebenkina L.A., Osipova E.V., Dolgikh M.I., Bolotova Ts.Ts. Process of lipid peroxidation in women of different ethnic groups with threatened miscarriage. Byull. VSNTs SO RAMN - Bulletin of Eastern-Siberian Scientific Center of Siberian Department of Russian Academy of Medical Sciences. 2010; 6 (76 ч. 2): 31–33.
  8. Kulikov V.Yu., Semenyuk A.V., Kolesnikova L.I. Perekisnoe okislenie lipidov i kholodovoi factor [Lipid peroxidation and cold shock]. Novosibirsk: Nauka. 1988. 192 p.
  9. Protopopova N.V., Kolesnikova L.I., Il'in V.P. Metabolizm i gemodinamika u beremennykh s arterial'noi gipertenziei [Metabolism and hemodynamics in pregnant women with arterial hypertension]. Novosibirsk: Nauka. 2000. 260 p.
  10. Suturina L.V., Kolesnikova L.I. Osnovnye patogeneticheskie mekhanizmy i metody korrektsii reproduktivnykh narushenii u bol'nykh s gipotalamicheskimi sindromami [The main pathogenetic mechanisms and methods of reproductive disorders correction in patients with hypothalamic syndromes]. Novosibirsk: Nauka. 2001. 134 p.
  11. Kolesnikova L.I., Osipova E.V., Grebenkina L.A. Okislitel'nyi stress pri reproduktivnykh narusheniyakh endokrinnogo geneza u zhenshchin [Oxidative stress in women with reproductive endocrine disorders]. Novosibirsk: Nauka. 2011. 116 p.
  12. Bouloumie A., Marumo T., Lafontan M., Busse R. Leptin induces oxidative stress in human endothelial cells. FASEB J. 1999; 13: 1231–1238.
  13. Ghezzi P., Bonetto V., Fratelli M. Thiol-disulfide balance: from the concept of oxidative stress to that of redox regulation. Antioxidants Redox Signaling.2005; 7 (8): 964–972.
  14. Volchek I.V., Petrov A.S. The possibility of personalized man therapy: 12-Year Experience. Terra Medica. 2010; 3: 3–10.
  15. Towsend D.M., Tew K.D., Tapiero H. The importance of glutathione in human disease. Biomed. Pharmacother. 2003; 57: 145–157.
  16. Kulinskii V.I., Kolesnichenko L.S. The biological role of glutathione. Uspekhi sovremennoi biologii = Achievements of Modern Biology. 1990; 110: 20–32.
  17. Kurilova L.S., Krutetskaya Z.I., Lebedev O.E., Antonov V.G. effect of oxidized glutathione and its pharmacological analogue, glutoxim, on intracellular Ca2+ concentration in macrophages. Tsitologiya = Сytology. 2008; 50: 452–461.
  18. Diaz-Montero C.M., Wang Y., Shao L., Feng W. et al. The glutatione disulfide mimetic NOV-002 inhibits cyclophosphamide-induced hematopoetic and immune supression by reducing oxidative stress. Free Radic. Biol. Med. 2012; 52: 1560–1568.
  19. Antonov V.G., Antushevich A.E., Burova E.B. et al. Possible mechanism of modulating effect of Glutoxim on regulatory effect of cytokines. Tsitokiny i vospalenie = Cytokines and Inflammation. 2005; 2: 72–73.
  20. Krutetskaya Z.I., Lebedev O.E., Krutetskaya N.I. i dr. The possible involvement of phosphoinositidekinases in action of oxidized glutathione and glutoxim on intracellular Ca2 + concentration in macrophages. Doklady Akademii nauk - Proceedings of the Russian Academy of Sciences. 2008; 422: 562–563.
  21. Bogush T.A., Dudko E.A., Bogush E.A., Kirsanov V.Yu., Antonov V.G. Glutoxim as an inhibitor of multiple drug resistance phenotype associated with Rgr expression. Antibiotiki i khimioterapiya = Antibiotics and Chemotherapy. 2010; 55: 5–6.
  22. Makhinas G.M., Zhukova G.M. Use of glutoxim in gastric cancer patients receiving platinum-containing chemotherapy. Onkologiya = Oncology. 2012; 1: 44–46.
  23. Chistyakov V.A. Non-specific defense mechanisms against destructive action of reactive oxygen species. Uspekhi sovremennoi biologii = Achievements of Modern Biology. 2008; 3: 300–306.

Statistics

Views

Abstract - 291

PDF (Russian) - 105

Cited-By


PlumX

Dimensions



Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies