DEVELOPMENT OF THE QUANTITATIVE IMMUNOCHROMATOGRAPHY TESTS FOR SOMATIC DEASEASE MARKERS DETECTION

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Abstract

The purpose of work was creation of quantative immunochromatographic tests (ICT)   for measuring concentrations of the marker substance associated with somatic diseases: immunoglobulin Е (IgE), C-reactive protein (CRP) and fibrin D-dimer  in blood serum (plasma),  which is carried out with the help of videodigital analyzers of domestic development "Reflecom" and "Zondaj". ICT were designed in "sandwich» format, using colloid gold and monoclonal antibodies. It is shown, that calibration curves received with the help of ICT and devices of videodigital registration, are well approximated by exponential dependence Y=Aexp(-x/B)+y0, where Y- device readings , х- analytes concentration, А, В, у0– constants. Samples of blood serum (plasma), under investigation received in conditions of a hospital from patients. Correlation with electrochemiluminescent immunoassay, enzyme linked immunoassay, latex-agglutinations assay and immunochromatographic method was observed.  Sensitivity of the developed tests was- 7,5 IU/ML for IgE, 5,7 ng/ml for CRP, 500 ng/ml for fibrin D-dimer. The developed analytical complex- videodigital analyzer "Zondaj" and ICT for quantitative measure of concentration IgE, fibrin D-dimer and CRP- can be successfully applied in  laboratory practice and clinical laboratory researches alongside with actual immunochemistry methods.

 

About the authors

S. I. Tret'yakov

State Scientific-Research Institute of Biological Engineering, FMBA, Moscow, Russian Federation

Author for correspondence.
Email: diasol@dol.ru
MD, leading research scientist of the department of spectral analysis methods of the Federal State Unitary Enterprise «State Scientific Research Institute of Biological Engineering» of the Federal Medical-Biology Agency. Address: build. 1, 75, Volokolamskoye Route, Moscow, 125424; tel.: (495) 491-86-83 Russian Federation

L. A. Basharova

State Scientific-Research Institute of Biological Engineering, FMBA, Moscow, Russian Federation

Email: diasol@dol.ru
MD, senior research scientist of the department of spectral analysis methods of the Federal State Unitary Enterprise «State Scientific Research Institute of Biological Engineering» of the Federal Medical-Biology Agency. Address: build. 1, 75, Volokolamskoye Route, Moscow, 125424; tel.: (495) 491-86-83 Russian Federation

S. P. Yarkov

State Scientific-Research Institute of Biological Engineering, FMBA, Moscow, Russian Federation

Email: diasol@dol.ru
PhD, head of the department of spectral analysis methods of the Federal State Unitary Enterprise «State Scientific Research Institute of Biological Engineering» of the Federal Medical-Biology Agency. Address: build. 1, 75, Volokolamskoye Route, Moscow, 125424; tel.: (495) 491-86-83 Russian Federation

A. A. Titov

State Scientific-Research Institute of Biological Engineering, FMBA, Moscow, Russian Federation

Email: diasol@dol.ru
junior research scientist of the department of spectral analysis methods of the Federal State Unitary Enterprise «State Scientific Research Institute of Biological Engineering» of the Federal Medical-Biology Agency. Address: build. 1, 75, Volokolamskoye Route, Moscow, 125424; tel.: (495) 491-86-83 Russian Federation

E. N. Khramov

State Scientific-Research Institute of Biological Engineering, FMBA, Moscow, Russian Federation

Email: niibp@dol.ru
PhD, director of the Federal State Unitary Enterprise «State Scientific Research Institute of Biological Engineering» of the Federal Medical-Biology Agency. Address: build. 1, 75, Volokolamskoye Route, Moscow, 125424; tel.: (495) 491-73-72 Russian Federation

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