ONCOLYTIC PROPERTIES OF SOME ORTHOPOXVIRUSES, ADENOVIRUSES AND PARVOVIRUSES IN HUMAN GLIOMA CELLS

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Abstract

Currently one of the most promising approaches in development of cancer virotherapy is based on the ability of oncolytic viruses to selective infection and lysis of tumor cells. Aim.The goal of the study was to identify and evaluate perspective oncolytic viruses capable of selectively destroying human glioma cells. Patients and methods. Original GB2m, GA14m and GB22m glioma cell cultures derived from patients were used for evaluating in vitro oncolytic activity of some typical orthopoxviruses, adenoviruses and parvoviruses. Results. The oncolytic activity in the human glioma cell models was confirmed for LIVP and WR strains of vaccinia virus,  Adel2 and Ad2del strains with deletions within E1B/55K gene and derived from human adenoviruses type 2 and 5, respectively. Conclusions. We consider these oncolytic viruses as promising agents for the treatment of human malignant glioma.

 

About the authors

I. A. Razumov

State Research Center of Virology and Biotechnology "Vector", Koltsovo, Novosibirsk Region; Russian Federation
Institute of Cytology and Genetics, SB RAS, Novosibirsk, Russian Federation

Author for correspondence.
Email: razumov@bionet.nsc.ru
PhD, leading research scientist of the Institute of Cytology and Genetics under Siberian Branch of the Russian Academy of Sciences (ICG SB RAS). Address: 10, Akademik Lavrent’ev Avenue, Novosibirsk, RF, 630090; tel.: +7 (383) 363-49-01 (add. 2208) Russian Federation

V. A. Svyatchenko

State Research Center of Virology and Biotechnology "Vector", Koltsovo, Novosibirsk Region; Russian Federation

Email: Svyatchenko@vector.nsc.ru
MD, Head of the Laboratory of State Research Center of Virology and Biotechnology “Vector”. Address: 10, SRCVB “Vector”, Koltsovo, Novosibirsk Region, RF, 630559; tel.: +7 (383) 363-47-53 Russian Federation

E. V. Protopopova

State Research Center of Virology and Biotechnology "Vector", Koltsovo, Novosibirsk Region; Russian Federation

Email: razumov@bionet.nsc.ru
MD, leading research scientist of State Research Center of Virology and Biotechnology “Vector”. Address: 10, SRCVB “Vector”, Koltsovo, Novosibirsk Region, RF, 630559; tel.: +7 (383) 363-47-53 Russian Federation

G. V. Kochneva

State Research Center of Virology and Biotechnology "Vector", Koltsovo, Novosibirsk Region; Russian Federation

Email: kochneva@vector.nsc.ru
PhD, Head of the Laboratory of State Research Center of Virology and Biotechnology “Vector”. Address: 10, SRCVB “Vector”, Koltsovo, Novosibirsk Region, RF, 630559; tel.: +7 (383) 363-47-53 Russian Federation

N. N. Kiselev

State Research Center of Virology and Biotechnology "Vector", Koltsovo, Novosibirsk Region; Russian Federation

Email: razumov@bionet.nsc.ru
senior research scientist of State Research Center of Virology and Biotechnology “Vector”. Address: 10, SRCVB “Vector”, Koltsovo, Novosibirsk Region, RF, 630559; tel.: +7 (383) 363-47-53 Russian Federation

N. V. Gubanova

Institute of Cytology and Genetics, SB RAS, Novosibirsk, Russian Federation

Email: nat@bionet.nsc.ru
MD, research scientist of the Institute of Cytology and Genetics under Siberian Branch of the Russian Academy of Sciences. Address: 10, Akademik Lavrent’ev Avenue, Novosibirsk, RF, 630090; tel.: +7 (383) 363-49-01 Russian Federation

A. G. Shilov

Institute of Cytology and Genetics, SB RAS, Novosibirsk, Russian Federation

Email: alxshilov@mail.ru
senior research scientist of the Institute of Cytology and Genetics under Siberian Branch of the Russian Academy of Sciences. Address: 10, Akademik Lavrent’ev Avenue, Novosibirsk, RF, 630090; tel.: +7 (383) 363-49-01 Russian Federation

V. A. Mordvinov

Institute of Cytology and Genetics, SB RAS, Novosibirsk, Russian Federation

Email: mordvin@bionet.nsc.ru
PhD, Deputy Director of the Institute of Cytology and Genetics under Siberian Branch of the Russian Academy of Sciences. Address: 10, Akademik Lavrent’ev Avenue, Novosibirsk, RF, 630090; tel.: +7 (383) 363-49-98 Russian Federation

S. V. Netesov

Novosibirsk State University, Russian Federation

Email: razumov@bionet.nsc.ru
professor, correspondent member of RAS, Vice-Principal of Novosibirsk State University. Address: 2, Pirogov Street, Novosibirsk, RF, 630090; tel.: +7 (383) 363-40-01 Russian Federation

P. M. Chumakov

Novosibirsk State University, Russian Federation
Engelhardt Institute of Molecular Biology, RAS, Moscow, Russian Federation

Email: chumakovpm@yahoo.com
PhD, professor, Head of the Laboratory of V.A. Engelhardt Institute of Molecular Biology of the Russian Academy of Sciences (IMB RAS). Address: 32, Vavilova Street, Moscow, RF, 119991; tel.: +7 (499) 135-23-31 Russian Federation

V. B. Loktev

State Research Center of Virology and Biotechnology "Vector", Koltsovo, Novosibirsk Region; Russian Federation
Institute of Cytology and Genetics, SB RAS, Novosibirsk, Russian Federation

Email: valeryloktev@gmail.com
PhD, professor, Head of the Department of State Research Center of Virology and Biotechnology “Vector”. Address: 10, SRCVB “Vector”, Koltsovo, Novosibirsk Region, RF, 630559; tel.: +7 (383) 363-47-53 Russian Federation

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