ONCOLYTIC PROPERTIES OF SOME ORTHOPOXVIRUSES, ADENOVIRUSES AND PARVOVIRUSES IN HUMAN GLIOMA CELLS

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Abstract


Currently one of the most promising approaches in development of cancer virotherapy is based on the ability of oncolytic viruses to selective infection and lysis of tumor cells. Aim.The goal of the study was to identify and evaluate perspective oncolytic viruses capable of selectively destroying human glioma cells. Patients and methods. Original GB2m, GA14m and GB22m glioma cell cultures derived from patients were used for evaluating in vitro oncolytic activity of some typical orthopoxviruses, adenoviruses and parvoviruses. Results. The oncolytic activity in the human glioma cell models was confirmed for LIVP and WR strains of vaccinia virus,  Adel2 and Ad2del strains with deletions within E1B/55K gene and derived from human adenoviruses type 2 and 5, respectively. Conclusions. We consider these oncolytic viruses as promising agents for the treatment of human malignant glioma.

 


About the authors

I. A. Razumov

State Research Center of Virology and Biotechnology "Vector", Koltsovo, Novosibirsk Region; Russian Federation
Institute of Cytology and Genetics, SB RAS, Novosibirsk, Russian Federation

Author for correspondence.
Email: razumov@bionet.nsc.ru

Russian Federation PhD, leading research scientist of the Institute of Cytology and Genetics under Siberian Branch of the Russian Academy of Sciences (ICG SB RAS). Address: 10, Akademik Lavrent’ev Avenue, Novosibirsk, RF, 630090; tel.: +7 (383) 363-49-01 (add. 2208)

V. A. Svyatchenko

State Research Center of Virology and Biotechnology "Vector", Koltsovo, Novosibirsk Region; Russian Federation

Email: Svyatchenko@vector.nsc.ru

Russian Federation MD, Head of the Laboratory of State Research Center of Virology and Biotechnology “Vector”. Address: 10, SRCVB “Vector”, Koltsovo, Novosibirsk Region, RF, 630559; tel.: +7 (383) 363-47-53

E. V. Protopopova

State Research Center of Virology and Biotechnology "Vector", Koltsovo, Novosibirsk Region; Russian Federation

Email: razumov@bionet.nsc.ru

Russian Federation MD, leading research scientist of State Research Center of Virology and Biotechnology “Vector”. Address: 10, SRCVB “Vector”, Koltsovo, Novosibirsk Region, RF, 630559; tel.: +7 (383) 363-47-53

G. V. Kochneva

State Research Center of Virology and Biotechnology "Vector", Koltsovo, Novosibirsk Region; Russian Federation

Email: kochneva@vector.nsc.ru

Russian Federation PhD, Head of the Laboratory of State Research Center of Virology and Biotechnology “Vector”. Address: 10, SRCVB “Vector”, Koltsovo, Novosibirsk Region, RF, 630559; tel.: +7 (383) 363-47-53

N. N. Kiselev

State Research Center of Virology and Biotechnology "Vector", Koltsovo, Novosibirsk Region; Russian Federation

Email: razumov@bionet.nsc.ru

Russian Federation senior research scientist of State Research Center of Virology and Biotechnology “Vector”. Address: 10, SRCVB “Vector”, Koltsovo, Novosibirsk Region, RF, 630559; tel.: +7 (383) 363-47-53

N. V. Gubanova

Institute of Cytology and Genetics, SB RAS, Novosibirsk, Russian Federation

Email: nat@bionet.nsc.ru

Russian Federation MD, research scientist of the Institute of Cytology and Genetics under Siberian Branch of the Russian Academy of Sciences. Address: 10, Akademik Lavrent’ev Avenue, Novosibirsk, RF, 630090; tel.: +7 (383) 363-49-01

A. G. Shilov

Institute of Cytology and Genetics, SB RAS, Novosibirsk, Russian Federation

Email: alxshilov@mail.ru

Russian Federation senior research scientist of the Institute of Cytology and Genetics under Siberian Branch of the Russian Academy of Sciences. Address: 10, Akademik Lavrent’ev Avenue, Novosibirsk, RF, 630090; tel.: +7 (383) 363-49-01

V. A. Mordvinov

Institute of Cytology and Genetics, SB RAS, Novosibirsk, Russian Federation

Email: mordvin@bionet.nsc.ru

Russian Federation PhD, Deputy Director of the Institute of Cytology and Genetics under Siberian Branch of the Russian Academy of Sciences. Address: 10, Akademik Lavrent’ev Avenue, Novosibirsk, RF, 630090; tel.: +7 (383) 363-49-98

S. V. Netesov

Novosibirsk State University, Russian Federation

Email: razumov@bionet.nsc.ru

Russian Federation professor, correspondent member of RAS, Vice-Principal of Novosibirsk State University. Address: 2, Pirogov Street, Novosibirsk, RF, 630090; tel.: +7 (383) 363-40-01

P. M. Chumakov

Novosibirsk State University, Russian Federation
Engelhardt Institute of Molecular Biology, RAS, Moscow, Russian Federation

Email: chumakovpm@yahoo.com

Russian Federation PhD, professor, Head of the Laboratory of V.A. Engelhardt Institute of Molecular Biology of the Russian Academy of Sciences (IMB RAS). Address: 32, Vavilova Street, Moscow, RF, 119991; tel.: +7 (499) 135-23-31

V. B. Loktev

State Research Center of Virology and Biotechnology "Vector", Koltsovo, Novosibirsk Region; Russian Federation
Institute of Cytology and Genetics, SB RAS, Novosibirsk, Russian Federation

Email: valeryloktev@gmail.com

Russian Federation PhD, professor, Head of the Department of State Research Center of Virology and Biotechnology “Vector”. Address: 10, SRCVB “Vector”, Koltsovo, Novosibirsk Region, RF, 630559; tel.: +7 (383) 363-47-53

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